College of Life Science, Hebei University, Baoding, China.
Desert Forest Experimental Center, Chinese Academy of Forestry, Dengkou, China.
Genome. 2019 Oct;62(10):689-703. doi: 10.1139/gen-2019-0024. Epub 2019 Jul 17.
Sea buckthorn ( L.) is a plant with economic and ecological value. It is uniquely capable of growing well under salt and drought stress. WRKY transcription factors play important roles in the ability of plants to resist stress. In this study, 48 genes were identified based on RNA sequencing of . Evaluation of expression pattern of , , , , , , , and suggested that they were involved in abiotic stress. Interestingly, , one of eight genes, was a positive regulator of abiotic stress tolerance in . In addition, most morphological attributes of roots in transgenic lines (overexpressing ) were also markedly increased compared with the wild-type (WT), including total lengths, specific root lengths, and surface areas. Stress tolerance of transgenic lines was also correlated with higher antioxidant activity (SOD and POD), lower percentage of relative conductivity (REC), and lower activity of malondialdehyde (MDA) under stress conditions. These findings represent a foundation of knowledge about the molecular mechanisms driving resistance to adverse conditions in plants; they are a promising step towards development of tree cultivars with improved tolerance to abiotic stress.
沙棘（ L.）是一种具有经济和生态价值的植物。它具有独特的耐盐耐旱能力。WRKY 转录因子在植物抵抗胁迫的能力中发挥着重要作用。本研究基于. 的 RNA 测序，鉴定了 48 个基因。对 、 、 、 、 、 、 的表达模式进行评估，表明它们参与了非生物胁迫。有趣的是，八个 基因之一的 ，是. 中耐非生物胁迫的正调控因子。此外，与野生型（WT）相比，过表达 基因的转基因 系的大多数根系形态特征也明显增加，包括总长度、比根长和表面积。在胁迫条件下，转基因系的耐受力也与较高的抗氧化活性（SOD 和 POD）、较低的相对电导率（REC）百分比和较低的丙二醛（MDA）活性相关。这些发现为研究植物对不良条件的抵抗机制提供了知识基础；这是朝着开发具有改善的非生物胁迫耐受性的树木品种迈出的有希望的一步。
