1 Department of General Surgery, Hebei Medical University Fourth Affiliated Hospital (Hebei Provincial Tumor Hospital), Shijiazhuang, Hebei, P.R. China.
2 Department of Gastrointestinal Surgery, Affiliated Hospital of Hebei University, Baoding, Hebei, P.R. China.
Technol Cancer Res Treat. 2019 Jan 1;18:1533033819858668. doi: 10.1177/1533033819858668.
To identify the expression of kinetochore scaffold 1 () in colorectal tumor tissues and to clarify the role of this gene in the proliferation capability of colorectal cancer cells.
A total of 108 paired colorectal tumor and normal tissue samples were collected from patients with colorectal cancer and subjected to quantitative polymerase chain reaction and immunohistochemistry analyses. Expression levels of KNL1 mRNA and protein were compared between tumor and normal tissues, and KNL1 levels were evaluated in relation to the patients' tumor differentiation, sex, lymph node metastasis, TNM stage, infiltration depth, age, and tumor location. Survival curves were also constructed and compared between patients with tumor samples with and without KLN1 protein expression. was under-expressed in colorectal cancer cells using lentiviral transfection with short hairpin RNA, and its function was evaluated by proliferation, colony-formation, and apoptosis assays. Expression levels of BUB1 protein were also compared between tumor and normal tissues, and the correlation between KNL1 expression and BUB1 expression in colorectal cancer tissues was examined.
KNL1 mRNA and protein were both highly expressed in colorectal tumor tissues compared with paired normal tissues. downregulation significantly inhibited colorectal cancer cell proliferation and colony formation, and promoted apoptosis. KNL1 protein expression was significantly associated with tumor differentiation, but not with sex, lymph node metastasis, TNM stage, infiltration depth, age, or tumor location. KNL1 protein expression was also significantly associated with poorer survival. Moreover, there was a significant correlation between KNL1 and BUB1 in colorectal cancer tissues.
KNL1 plays an effective role in decreasing apoptosis and promoting the proliferation of colorectal cancer cells, suggesting that its inhibition may represent a promising therapeutic approach in patients with colorectal cancer.
鉴定动粒支架 1（）在结直肠肿瘤组织中的表达，并阐明该基因在结直肠癌细胞增殖能力中的作用。
收集 108 对结直肠癌患者的结直肠肿瘤和正常组织标本，进行实时定量聚合酶链反应和免疫组织化学分析。比较肿瘤和正常组织中 KNL1 mRNA 和蛋白的表达水平，并评估 KNL1 水平与患者肿瘤分化、性别、淋巴结转移、TNM 分期、浸润深度、年龄和肿瘤位置的关系。还构建了生存曲线，并比较了有和没有 KLN1 蛋白表达的肿瘤样本患者之间的生存曲线。使用短发夹 RNA 慢病毒转染使结直肠癌细胞中的表达下调，并通过增殖、集落形成和凋亡测定评估其功能。还比较了肿瘤和正常组织中 BUB1 蛋白的表达水平，并检测了结直肠癌细胞中 KNL1 表达与 BUB1 表达之间的相关性。
与配对的正常组织相比，结直肠肿瘤组织中 KNL1 mRNA 和蛋白表达均明显升高。下调表达显著抑制结直肠癌细胞增殖和集落形成，并促进细胞凋亡。KNL1 蛋白表达与肿瘤分化显著相关，但与性别、淋巴结转移、TNM 分期、浸润深度、年龄或肿瘤位置无关。KNL1 蛋白表达与较差的生存显著相关。此外，结直肠癌细胞中 KNL1 与 BUB1 之间存在显著相关性。
KNL1 在降低凋亡和促进结直肠癌细胞增殖方面发挥了有效作用，提示抑制其表达可能是结直肠癌患者有前途的治疗方法。
