Max-Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany
Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK.
J Cell Biol. 2019 Aug 5;218(8):2762-2781. doi: 10.1083/jcb.201809121. Epub 2019 Jul 17.
Epithelial organ size and shape depend on cell shape changes, cell-matrix communication, and apical membrane growth. The embryonic tracheal network is an excellent model to study these processes. Here, we show that the transcriptional coactivator of the Hippo pathway, Yorkie (YAP/TAZ in vertebrates), plays distinct roles in the developing airways. Yorkie exerts a cytoplasmic function by binding Twinstar, the orthologue of the vertebrate actin-severing protein Cofilin, to regulate F-actin levels and apical cell membrane size, which are required for proper tracheal tube elongation. Second, Yorkie controls water tightness of tracheal tubes by transcriptional regulation of the gene (). We conclude that Yorkie has a dual role in tracheal development to ensure proper tracheal growth and functionality.
上皮器官的大小和形状取决于细胞形状的变化、细胞-基质的通讯和顶膜的生长。胚胎气管网络是研究这些过程的极好模型。在这里，我们表明 Hippo 通路的转录共激活因子 Yorkie（脊椎动物中的 YAP/TAZ）在发育中的气道中发挥着不同的作用。Yorkie 通过与脊椎动物肌动蛋白切割蛋白 Cofilin 的同源物 Twinstar 结合，发挥细胞质功能，以调节 F-肌动蛋白水平和顶膜大小，这是适当的气管管伸长所必需的。其次，Yorkie 通过对基因（）的转录调控控制气管管的水密性。我们得出结论，Yorkie 在气管发育中具有双重作用，以确保气管的正常生长和功能。
