Liu Shengjie, Fu Hua, Jiang Jieming, Chen Zhongjian, Gao Jiadong, Shu Haoran, Zhang Sheng, Yang Chengwei, Liu Jun
Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou, China.
Front Plant Sci. 2019 Jun 27;10:848. doi: 10.3389/fpls.2019.00848. eCollection 2019.
Glutaredoxins (Grxs) are a ubiquitous group of oxidoreductase enzymes that are important in plant growth and development; however, the functions of rice Grxs have not been fully elucidated. In this paper, we showed that one of the Grxs, encoded by , exhibited Grx activity. Furthermore, we demonstrated that was able to regulate embryo development during embryogenesis. Transgenic rice lines overexpressing unexpectedly exhibited degenerate embryos as well as embryoless seeds. Our data indicated that the embryonic abnormalities occurred at an early stage during embryogenesis. We found that the expression of several endodermal layer marker genes for embryo development, such as (apical region marker), (L2 ground tissue marker), and (L3 vascular tissue marker), were significantly decreased in the overexpressed transgenic rice lines. In contrast, the transcript levels of the majority of protodermal layer markers, including , , , and , and the shoot apical meristem marker , showed little change between the wild-type (WT) and -overexpressing embryos. Surprisingly, the seed weight of the overexpressed transgenic rice was remarkably increased in comparison to that of the WT. These results indicate that the overexpression of interferes with the normal embryogenesis of rice embryos and leads to increased grain weight. To the best of our knowledge, this is the first report that is a rice embryo development-associated gene.
谷氧还蛋白（Grxs）是一类普遍存在的氧化还原酶，在植物生长发育中起重要作用；然而，水稻谷氧还蛋白的功能尚未完全阐明。在本文中，我们发现由 编码的一种谷氧还蛋白具有谷氧还蛋白活性。此外，我们证明了 在胚胎发生过程中能够调节胚胎发育。意外的是，过表达 的转基因水稻品系表现出胚胎退化以及无胚种子。我们的数据表明胚胎异常发生在胚胎发生的早期阶段。我们发现一些用于胚胎发育的内胚层标记基因的表达，如 （顶端区域标记）、 （L2基本组织标记）和 （L3维管组织标记），在过表达的转基因水稻品系中显著降低。相比之下，大多数原表皮层标记基因的转录水平，包括 、 、 和 ，以及茎尖分生组织标记基因 ，在野生型（WT）和过表达 的胚胎之间变化不大。令人惊讶的是，与野生型相比，过表达的转基因水稻种子重量显著增加。这些结果表明， 的过表达干扰了水稻胚胎的正常胚胎发生并导致粒重增加。据我们所知，这是首次报道 是一个与水稻胚胎发育相关的基因。
