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水稻谷氧还蛋白OsGrx_C7和OsGrx_C2.1的过表达降低了拟南芥细胞内砷的积累并提高了其耐受性。

Overexpression of Rice Glutaredoxin OsGrx_C7 and OsGrx_C2.1 Reduces Intracellular Arsenic Accumulation and Increases Tolerance in Arabidopsis thaliana.

作者信息

Verma Pankaj K, Verma Shikha, Pande Veena, Mallick Shekhar, Deo Tripathi Rudra, Dhankher Om P, Chakrabarty Debasis

机构信息

Genetics and Molecular Biology Division, Council of Scientific and Industrial Research-National Botanical Research InstituteLucknow, India; Department of Biotechnology, Kumaun UniversityNainital, India.

Department of Biotechnology, Kumaun University Nainital, India.

出版信息

Front Plant Sci. 2016 Jun 1;7:740. doi: 10.3389/fpls.2016.00740. eCollection 2016.

DOI:10.3389/fpls.2016.00740
PMID:27313586
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4887470/
Abstract

Glutaredoxins (Grxs) are a family of small multifunctional proteins involved in various cellular functions, including redox regulation and protection under oxidative stress. Despite the high number of Grx genes in plant genomes (48 Grxs in rice), the biological functions and physiological roles of most of them remain unknown. Here, the functional characterization of the two arsenic-responsive rice Grx family proteins, OsGrx_C7 and OsGrx_C2.1 are reported. Over-expression of OsGrx_C7 and OsGrx_C2.1 in transgenic Arabidopsis thaliana conferred arsenic (As) tolerance as reflected by germination, root growth assay, and whole plant growth. Also, the transgenic expression of OsGrxs displayed significantly reduced As accumulation in A. thaliana seeds and shoot tissues compared to WT plants during both AsIII and AsV stress. Thus, OsGrx_C7 and OsGrx_C2.1 seem to be an important determinant of As-stress response in plants. OsGrx_C7 and OsGrx_C2.1 transgenic showed to maintain intracellular GSH pool and involved in lowering AsIII accumulation either by extrusion or reducing uptake by altering the transcript of A. thaliana AtNIPs. Overall, OsGrx_C7 and OsGrx_C2.1 may represent a Grx family protein involved in As stress response and may allow a better understanding of the As induced stress pathways and the design of strategies for the improvement of stress tolerance as well as decreased As content in crops.

摘要

谷氧还蛋白（Grxs）是一类参与多种细胞功能的小分子量多功能蛋白质，包括氧化还原调节和氧化应激下的保护作用。尽管植物基因组中谷氧还蛋白基因数量众多（水稻中有48个谷氧还蛋白），但其中大多数的生物学功能和生理作用仍不清楚。本文报道了两种对砷有响应的水稻谷氧还蛋白家族蛋白OsGrx_C7和OsGrx_C2.1的功能特性。通过发芽、根生长测定和整株植物生长情况反映出，在转基因拟南芥中过表达OsGrx_C7和OsGrx_C2.1可赋予其对砷（As）的耐受性。此外，在亚砷酸盐（AsIII）和砷酸盐（AsV）胁迫期间，与野生型植物相比，OsGrxs的转基因表达使拟南芥种子和地上组织中的砷积累显著减少。因此，OsGrx_C7和OsGrx_C2.1似乎是植物砷胁迫响应的重要决定因素。OsGrx_C7和OsGrx_C2.1转基因植株显示能维持细胞内谷胱甘肽（GSH）库，并通过改变拟南芥水通道蛋白NIPs（AtNIPs）的转录本，以排出或减少吸收的方式参与降低AsIII的积累。总体而言，OsGrx_C7和OsGrx_C2.1可能代表了参与砷胁迫响应的谷氧还蛋白家族蛋白，可能有助于更好地理解砷诱导的胁迫途径，以及设计提高胁迫耐受性和降低作物砷含量的策略。

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