[同源DNA序列介导的质粒整合入铜绿假单胞菌染色体]
[Integration of a plasmid into the Pseudomonas aeruginosa chromosome mediated by homologous DNA sequences].
作者信息
Astashkin E I, Pachkunov D M, Kiselev V I
出版信息
Mol Gen Mikrobiol Virusol. 1988 Feb(2):21-3.
PMID:3131670
Abstract
The integrative vectors for Pseudomonas aeruginosa cells were constructed on the basis of the plasmid pSUP202. To construct the integrative vectors the fragments of chromosomal DNA mediating the homologous recombination were cloned in the plasmid. The possibility of cloning of different genes in the chromosomes of Pseudomonas aeruginosa cells was illustrated by KmR gene cloning.
摘要
基于质粒pSUP202构建了铜绿假单胞菌细胞的整合载体。为构建整合载体，将介导同源重组的染色体DNA片段克隆到质粒中。通过卡那霉素抗性基因（KmR基因）克隆证明了在铜绿假单胞菌细胞染色体中克隆不同基因的可能性。
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