Ardila Héctor Javier, Sanabria-Salas María Carolina, Meneses Ximena, Rios Rafael, Huertas-Salgado Antonio, Serrano Martha Lucía
Grupo de Investigación en Biología del Cáncer, Instituto Nacional de Cancerología, Bogotá, Colombia.
Instituto de Genética, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
Mol Clin Oncol. 2019 Aug;11(2):201-207. doi: 10.3892/mco.2019.1876. Epub 2019 Jun 11.
Colorectal cancer (CRC) is one of the prominent causes of cancer related deaths because, in part, there is not an early, non-invasive, effective detection strategy. Circulating microRNAs (miRNAs) have been proposed as potential non-invasive biomarkers for CRC. In this study, we evaluated the miRNA profile in sixteen CRC tissues by Next-Generation-Sequencing and compared the circulating expression levels of 22 miRNAs among 45 CRC, 14 hyperplastic polyps, 11 advanced adenoma patients and 45 control subjects, by reverse transcription-quantitative PCR, to search for miRNAs which could be potential biomarkers. In total, nine of them represented 70% of total read counts (miR-10a-5p, miR-192-5p, miR-10b-5p, miR-22-3p, miR-26a-5p, miR-148a-3p, miR-181a-5p, miR-92a-3p and miR-143-5p). analysis found eight candidates to mature miRNAs. With respect to circulating miRNA, we found higher serum expression levels of miR-143-3p, miR-141-3p and miR-200c-3p in the CRC and adenoma groups compared with controls (P<0.002), and we also found significant higher levels of miR-141-3p and miR-200c-3p in serum of adenoma patients compared with the CRC group. In conclusion, the measurement of miRNAs in the blood could complement current screening methods for CRC and might provide new insights into mechanisms of tumorigenesis. miR-143-3p, miR-141-3p and miR-200c-3p could be interesting miRNAs to study as potential biomarkers for CRC.
结直肠癌（CRC）是癌症相关死亡的主要原因之一，部分原因是缺乏早期、非侵入性的有效检测策略。循环微小RNA（miRNA）已被提议作为CRC潜在的非侵入性生物标志物。在本研究中，我们通过下一代测序评估了16个CRC组织中的miRNA谱，并通过逆转录定量PCR比较了45例CRC患者、14例增生性息肉患者、11例高级别腺瘤患者和45例对照受试者中22种miRNA的循环表达水平，以寻找可能的生物标志物miRNA。其中，9种miRNA占总读数的70%（miR-10a-5p、miR-192-5p、miR-10b-5p、miR-22-3p、miR-26a-5p、miR-148a-3p、miR-181a-5p、miR-92a-3p和miR-143-5p）。分析发现8个候选成熟miRNA。关于循环miRNA，我们发现CRC组和腺瘤组血清中miR-143-3p、miR-141-3p和miR-200c-3p的表达水平高于对照组（P<0.002），并且我们还发现腺瘤患者血清中miR-141-3p和miR-200c-3p的水平显著高于CRC组。总之，血液中miRNA的检测可以补充目前CRC的筛查方法，并可能为肿瘤发生机制提供新的见解。miR-143-3p、miR-141-3p和miR-200c-3p可能是作为CRC潜在生物标志物进行研究的有趣miRNA。
