Department of Research, Guangxi Medical University Cancer Hospital, No. 71 Hedi Road, Nanning, 530021, China.
Department of Gastrointestinal Surgery, Third Affiliated Hospital of Guangxi Medical University, Nanning, 530031, China.
Mol Biol Rep. 2023 Sep;50(9):7253-7261. doi: 10.1007/s11033-023-08642-8. Epub 2023 Jul 7.
Aberrant expression of miRNAs have been implicated in cancers, but the role of miRNAs in colorectal cancer (CRC) remains need to be elucidated. This study aimed to identify miRNAs that related to colorectal cancer (CRC) pathogenesis and determine the diagnostic value.
Three GEO datasets (GSE128449, GSE35602 and GSE49246) with 131 samples were used to screen miRNAs that differential expression between tumor and control tissues. The expression of the identified miRNAs was validated in 50 clinical tissue samples and the GSE35834 dataset. The clinical significance of these miRNAs was analyzed in the TCGA dataset and clinical tissue samples. The expression of miRNAs in tissues and plasma samples were tested by RT-PCR assay in clinical samples, and their diagnostic value was determined.
The analysis of three GEO datasets revealed that miR-595 and miR-1237 were upregulated, while miR-126, miR-139, and miR-143 were downregulated in CRC tissues compared to control tissues. The differential expression of the five miRNAs in CRC tissues was confirmed using clinical tissue samples and GEO databases. There was no significant correlation between the TNM stage and tumor stage of CRC and any of the five miRNAs. Plasma expression of the miRNAs differed significantly between CRC and non-cancer patients, and each miRNA had moderate diagnostic value for CRC. Combining the five miRNAs provided better diagnostic potential for CRC than a single miRNA.
This study demonstrated that five miRNAs were related to the pathogenesis of CRC, but independent of the stage of CRC; Plasma expression of these miRNAs have moderate diagnostic value, and combination of these miRNAs showed better diagnostic ability in CRC.
miRNA 的异常表达与癌症有关，但 miRNA 在结直肠癌 (CRC) 中的作用仍需阐明。本研究旨在鉴定与结直肠癌 (CRC) 发病机制相关的 miRNA，并确定其诊断价值。
使用三个 GEO 数据集（GSE128449、GSE35602 和 GSE49246），其中包含 131 个样本，筛选肿瘤组织与对照组织之间差异表达的 miRNA。在 50 个临床组织样本和 GSE35834 数据集验证了鉴定的 miRNA 的表达。在 TCGA 数据集和临床组织样本中分析了这些 miRNA 的临床意义。在临床样本中通过 RT-PCR 检测 miRNA 在组织和血浆样本中的表达，并确定其诊断价值。
三个 GEO 数据集的分析表明，与对照组织相比，miR-595 和 miR-1237 在 CRC 组织中上调，而 miR-126、miR-139 和 miR-143 下调。使用临床组织样本和 GEO 数据库验证了这 5 个 miRNA 在 CRC 组织中的差异表达。这 5 个 miRNA 与 CRC 的 TNM 分期和肿瘤分期之间没有显著相关性。CRC 患者与非癌症患者的血浆 miRNA 表达差异显著，每个 miRNA 对 CRC 均具有中等诊断价值。将 5 个 miRNA 结合起来，比单个 miRNA 对 CRC 具有更好的诊断潜力。
本研究表明，这 5 个 miRNA 与 CRC 的发病机制有关，但与 CRC 的分期无关；这些 miRNA 的血浆表达具有中等诊断价值，将这些 miRNA 结合起来可以提高 CRC 的诊断能力。
