Paik Inyup, Huq Enamul
Department of Molecular Biosciences, The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX, USA.
Biological Laboratories, University of Texas at Austin, Austin, TX, USA.
Methods Mol Biol. 2019;2026:21-28. doi: 10.1007/978-1-4939-9612-4_2.
Phytochromes are red/far-red light receptors that perceive and transduce light signals to regulate various physiological responses. A large number of phytochrome interacting proteins have been identified using genetic and biochemical approaches. A direct interaction between phytochrome and its interacting proteins, therefore, defines one of the critical steps to initiate light signaling cascades in plants. Thus it is important to thoroughly examine the light-dependent interaction between phytochromes and putative phytochrome-interacting proteins. In this chapter, a protocol for rapid and simple light dependent in vitro coimmunoprecipitation between phytochromes and phytochrome interacting factors is described. In principle, this protocol can be adapted for other putative phytochrome interacting proteins.
光敏色素是感受并转导光信号以调节各种生理反应的红光/远红光受体。利用遗传学和生物化学方法已鉴定出大量光敏色素相互作用蛋白。因此，光敏色素与其相互作用蛋白之间的直接相互作用定义了植物中启动光信号级联反应的关键步骤之一。因此，全面研究光敏色素与假定的光敏色素相互作用蛋白之间的光依赖性相互作用非常重要。在本章中，描述了一种用于光敏色素与光敏色素相互作用因子之间快速、简单的光依赖性体外免疫共沉淀的方法。原则上，该方法可适用于其他假定的光敏色素相互作用蛋白。
