一种相互确保破坏机制减弱了拟南芥中的光信号传导。
A mutually assured destruction mechanism attenuates light signaling in Arabidopsis.
作者信息
Ni Weimin, Xu Shou-Ling, Tepperman James M, Stanley David J, Maltby Dave A, Gross John D, Burlingame Alma L, Wang Zhi-Yong, Quail Peter H
机构信息
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720 and U.S. Department of Agriculture/Agriculture Research Service, Plant Gene Expression Center, Albany, CA 94710.
Department of Pharmaceutical Chemistry, University of California, San Francisco, CA 94143.
出版信息
Science. 2014 Jun 6;344(6188):1160-1164. doi: 10.1126/science.1250778.
Abstract
After light-induced nuclear translocation, phytochrome photoreceptors interact with and induce rapid phosphorylation and degradation of basic helix-loop-helix transcription factors, such as PHYTOCHROME-INTERACTING FACTOR 3 (PIF3), to regulate gene expression. Concomitantly, this interaction triggers feedback reduction of phytochrome B (phyB) levels. Light-induced phosphorylation of PIF3 is necessary for the degradation of both proteins. We report that this PIF3 phosphorylation induces, and is necessary for, recruitment of LRB [Light-Response Bric-a-Brack/Tramtrack/Broad (BTB)] E3 ubiquitin ligases to the PIF3-phyB complex. The recruited LRBs promote concurrent polyubiqutination and degradation of both PIF3 and phyB in vivo. These data reveal a linked signal-transmission and attenuation mechanism involving mutually assured destruction of the receptor and its immediate signaling partner.
摘要
光诱导核转位后，光敏色素光受体与碱性螺旋-环-螺旋转录因子相互作用并诱导其快速磷酸化和降解，如光敏色素相互作用因子3（PIF3），以调节基因表达。同时，这种相互作用触发光敏色素B（phyB）水平的反馈降低。PIF3的光诱导磷酸化是两种蛋白质降解所必需的。我们报道，这种PIF3磷酸化诱导LRB[光响应Bric-a-Brack/Tramtrack/Broad（BTB）]E3泛素连接酶募集到PIF3-phyB复合物中，并且是其必需条件。募集到的LRB在体内促进PIF3和phyB同时多聚泛素化和降解。这些数据揭示了一种连锁的信号传递和衰减机制，涉及受体与其直接信号伴侣的相互确保破坏。
相似文献
1
A mutually assured destruction mechanism attenuates light signaling in Arabidopsis.一种相互确保破坏机制减弱了拟南芥中的光信号传导。
Science. 2014 Jun 6;344(6188):1160-1164. doi: 10.1126/science.1250778.
2
Multisite light-induced phosphorylation of the transcription factor PIF3 is necessary for both its rapid degradation and concomitant negative feedback modulation of photoreceptor phyB levels in Arabidopsis.多部位光诱导的转录因子 PIF3 的磷酸化对于其快速降解及其在拟南芥中对光受体 phyB 水平的负反馈调节都是必需的。
Plant Cell. 2013 Jul;25(7):2679-98. doi: 10.1105/tpc.113.112342. Epub 2013 Jul 31.
3
PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3.PPKs 将来自光敏色素光受体的直接信号转导到转录因子 PIF3。
Nat Commun. 2017 May 11;8:15236. doi: 10.1038/ncomms15236.
4
Light-Dependent Degradation of PIF3 by SCF Promotes a Photomorphogenic Response in Arabidopsis.光依赖的 PIF3 降解通过 SCF 促进拟南芥的光形态建成反应。
Curr Biol. 2017 Aug 21;27(16):2420-2430.e6. doi: 10.1016/j.cub.2017.06.062. Epub 2017 Jul 20.
5
Phytochrome B Induces Intron Retention and Translational Inhibition of PHYTOCHROME-INTERACTING FACTOR3.光敏色素 B 诱导 PHYTOCHROME-INTERACTING FACTOR3 内含子保留和翻译抑制。
Plant Physiol. 2020 Jan;182(1):159-166. doi: 10.1104/pp.19.00835. Epub 2019 Nov 5.
6
Phytochrome induces rapid PIF5 phosphorylation and degradation in response to red-light activation.光敏色素在红光激活后诱导PIF5快速磷酸化和降解。
Plant Physiol. 2007 Nov;145(3):1043-51. doi: 10.1104/pp.107.105601. Epub 2007 Sep 7.
7
Phytochrome B and AGB1 Coordinately Regulate Photomorphogenesis by Antagonistically Modulating PIF3 Stability in Arabidopsis.光敏色素 B 和 AGB1 通过拮抗调节 PIF3 的稳定性来协调调控拟南芥的光形态建成。
Mol Plant. 2019 Feb 4;12(2):229-247. doi: 10.1016/j.molp.2018.12.003. Epub 2018 Dec 18.
8
Photoactivated phytochrome induces rapid PIF3 phosphorylation prior to proteasome-mediated degradation.光激活的光敏色素在蛋白酶体介导的降解之前诱导PIF3快速磷酸化。
Mol Cell. 2006 Aug 4;23(3):439-46. doi: 10.1016/j.molcel.2006.06.011.
9
The Arabidopsis phytochrome-interacting factor PIF7, together with PIF3 and PIF4, regulates responses to prolonged red light by modulating phyB levels.拟南芥光敏色素相互作用因子PIF7与PIF3和PIF4一起，通过调节phyB水平来调控对长时间红光的反应。
Plant Cell. 2008 Feb;20(2):337-52. doi: 10.1105/tpc.107.052142. Epub 2008 Feb 5.
10
14-3-3 proteins regulate photomorphogenesis by facilitating light-induced degradation of PIF3.14-3-3 蛋白通过促进光诱导的 PIF3 降解来调节光形态建成。
New Phytol. 2023 Jan;237(1):140-159. doi: 10.1111/nph.18494. Epub 2022 Sep 30.
引用本文的文献
1
Transcriptomic and Metabolomic Insights into Plant Hormone Modulation and Secondary Metabolite Accumulation in Basil Under Far-Red and Ultraviolet-A Light.转录组学和代谢组学揭示远红光和紫外线-A光对罗勒中植物激素调节和次生代谢产物积累的影响
Int J Mol Sci. 2025 Apr 16;26(8):3758. doi: 10.3390/ijms26083758.
2
Both phytochrome A and phyB interact with PHYTOCHROME-INTERACTING FACTORs through an evolutionary conserved phy-APA interaction.光敏色素A和光敏色素B都通过一种进化保守的phy-APA相互作用与光敏色素互作因子相互作用。
Nat Commun. 2025 Apr 26;16(1):3946. doi: 10.1038/s41467-025-59327-8.
3
The demethylase REF6 physically interacts with phyB to promote hypocotyl elongation under red light.去甲基化酶REF6与phyB发生物理相互作用，以促进红光下的下胚轴伸长。
Proc Natl Acad Sci U S A. 2025 Mar 18;122(11):e2417253122. doi: 10.1073/pnas.2417253122. Epub 2025 Mar 10.
4
The evening complex component ELF3 recruits H3K4me3 demethylases to repress PHYTOCHROME INTERACTING FACTOR4 and 5 in Arabidopsis.晚间复合体组分ELF3招募H3K4me3去甲基化酶以抑制拟南芥中的光敏色素互作因子4和5。
Plant Cell. 2024 Dec 23;37(1). doi: 10.1093/plcell/koaf014.
5
NPR1 promotes blue light-induced plant photomorphogenesis by ubiquitinating and degrading PIF4.NPR1通过泛素化和降解PIF4来促进蓝光诱导的植物光形态建成。
Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2412755121. doi: 10.1073/pnas.2412755121. Epub 2024 Dec 19.
6
A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation.生物钟的输出功能独立于 phyB 来维持白天 PIF3 的降解。
Proc Natl Acad Sci U S A. 2024 Aug 27;121(35):e2408322121. doi: 10.1073/pnas.2408322121. Epub 2024 Aug 20.
7
Phytochrome C and Low Temperature Promote the Protein Accumulation and Red-Light Signaling of Phytochrome D.光敏色素 C 和低温促进光敏色素 D 的蛋白积累和红光信号转导。
Plant Cell Physiol. 2024 Nov 13;65(10):1717-1735. doi: 10.1093/pcp/pcae089.
8
MPK4-mediated phosphorylation of PHYTOCHROME INTERACTING FACTOR4 controls thermosensing by regulating histone variant H2A.Z deposition.MPK4 介导的 PHYTOCHROME INTERACTING FACTOR4 的磷酸化通过调控组蛋白变体 H2A.Z 的沉积来控制热感。
Plant Cell. 2024 Oct 3;36(10):4535-4556. doi: 10.1093/plcell/koae223.
9
PHOSPHATASE 2A dephosphorylates PHYTOCHROME-INTERACTING FACTOR3 to modulate photomorphogenesis in Arabidopsis.磷酸酶 2A 去磷酸化 PHYTOCHROME-INTERACTING FACTOR3 以调节拟南芥的光形态建成。
Plant Cell. 2024 Oct 3;36(10):4457-4471. doi: 10.1093/plcell/koae200.
10
The lowdown on breakdown: Open questions in plant proteolysis.植物蛋白水解：研究现状与未解之谜。
Plant Cell. 2024 Sep 3;36(9):2931-2975. doi: 10.1093/plcell/koae193.
本文引用的文献
1
Multisite light-induced phosphorylation of the transcription factor PIF3 is necessary for both its rapid degradation and concomitant negative feedback modulation of photoreceptor phyB levels in Arabidopsis.多部位光诱导的转录因子 PIF3 的磷酸化对于其快速降解及其在拟南芥中对光受体 phyB 水平的负反馈调节都是必需的。
Plant Cell. 2013 Jul;25(7):2679-98. doi: 10.1105/tpc.113.112342. Epub 2013 Jul 31.
2
A quartet of PIF bHLH factors provides a transcriptionally centered signaling hub that regulates seedling morphogenesis through differential expression-patterning of shared target genes in Arabidopsis.四个 PIF bHLH 因子提供了一个转录中心信号枢纽，通过在拟南芥中共享靶基因的差异表达模式调节幼苗形态发生。
PLoS Genet. 2013;9(1):e1003244. doi: 10.1371/journal.pgen.1003244. Epub 2013 Jan 31.
3
The light-response BTB1 and BTB2 proteins assemble nuclear ubiquitin ligases that modify phytochrome B and D signaling in Arabidopsis.光响应 BTB1 和 BTB2 蛋白组装核泛素连接酶，可修饰拟南芥中的光敏色素 B 和 D 信号。
Plant Physiol. 2012 Sep;160(1):118-34. doi: 10.1104/pp.112.199109. Epub 2012 Jun 25.
4
Phytochrome signaling in green Arabidopsis seedlings: impact assessment of a mutually negative phyB-PIF feedback loop.在绿色拟南芥幼苗中，光敏色素信号传导：相互负反馈的 phyB-PIF 反馈回路的影响评估。
Mol Plant. 2012 May;5(3):734-49. doi: 10.1093/mp/sss031. Epub 2012 Apr 5.
5
Composite low affinity interactions dictate recognition of the cyclin-dependent kinase inhibitor Sic1 by the SCFCdc4 ubiquitin ligase.复合低亲和力相互作用决定了细胞周期蛋白依赖性激酶抑制剂 Sic1 被 SCFCdc4 泛素连接酶识别。
Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3287-92. doi: 10.1073/pnas.1116455109. Epub 2012 Feb 10.
6
The cullin-RING ubiquitin-protein ligases.Cullin-RING 泛素连接酶。
Annu Rev Plant Biol. 2011;62:299-334. doi: 10.1146/annurev-arplant-042809-112256.
7
Feedback regulation of EGFR signalling: decision making by early and delayed loops.EGFR 信号反馈调节：早期和延迟反馈环的决策。
Nat Rev Mol Cell Biol. 2011 Feb;12(2):104-17. doi: 10.1038/nrm3048.
8
The biological framework: translational research from bench to clinic.生物学框架：从实验室到临床的转化研究。
Oncologist. 2010;15 Suppl 5:1-7. doi: 10.1634/theoncologist.2010-S5-01.
9
PIFs: pivotal components in a cellular signaling hub.PIFs：细胞信号枢纽中的关键组成部分。
Trends Plant Sci. 2011 Jan;16(1):19-28. doi: 10.1016/j.tplants.2010.08.003. Epub 2010 Sep 20.
10
Arabidopsis PHYTOCHROME INTERACTING FACTOR proteins promote phytochrome B polyubiquitination by COP1 E3 ligase in the nucleus.拟南芥光受体相互作用因子蛋白在核内通过 COP1 E3 连接酶促进光敏色素 B 的多泛素化。
Plant Cell. 2010 Jul;22(7):2370-83. doi: 10.1105/tpc.109.072520. Epub 2010 Jul 6.
Zotero 插件