Terry Matthew J, Kacprzak Sylwia M
School of Biological Sciences, University of Southampton, Southampton, UK.
Methods Mol Biol. 2019;2026:169-177. doi: 10.1007/978-1-4939-9612-4_14.
Etiolated seedlings accumulate the chlorophyll biosynthesis intermediate protochlorophyllide (Pchlide) and measuring Pchlide can be important for characterizing photomorphogenic mutants that may be affected in chloroplast development. In this chapter we outline a simple and sensitive method for quantifying Pchlide in extracts of Arabidopsis seedlings using fluorescence spectroscopy. This method can be easily adapted to study chloroplast development in a wide range of plant species.
黄化幼苗会积累叶绿素生物合成中间体原叶绿素酸酯（Pchlide），测定Pchlide对于鉴定可能在叶绿体发育中受到影响的光形态建成突变体很重要。在本章中，我们概述了一种使用荧光光谱法定量拟南芥幼苗提取物中Pchlide的简单而灵敏的方法。该方法可以很容易地适用于研究多种植物物种的叶绿体发育。
