Ermert Anna Lena, Stahl Fabian, Gans Tanja, Hughes Jon
Institute for Plant Physiology, Justus Liebig University, Giessen, Germany.
Methods Mol Biol. 2019;2026:225-236. doi: 10.1007/978-1-4939-9612-4_19.
In mosses such as Physcomitrella patens phytochrome photoreceptors steer directional/vectorial responses to unilateral/polarized light. In this chapter, we describe procedures to assay phototropism and polarotropism quantitatively in wild type and mutant lines. Protonemata are placed on agar-based medium in square Petri dishes in darkness for 1 week, allowing caulonemata to develop and grow negatively gravitropically. For phototropism, the dishes are placed vertically in black boxes and unilaterally irradiated with continuous red light. For polarotropism, Petri dishes are placed horizontally and irradiated with linearly polarized red light from above. After irradiation, the filaments are photographed using a macroscope with CCD camera and the bending angles measured using image processing software. The data are transfered to a spreadsheet program, placed into 10° bending angle classes and illustrated using a circular histogram.
在诸如小立碗藓（Physcomitrella patens）等苔藓植物中，光敏色素光感受器引导对单侧/偏振光的定向/矢量响应。在本章中，我们描述了在野生型和突变株系中定量测定向光性和向极性的方法。将原丝体置于方形培养皿中基于琼脂的培养基上，在黑暗中放置1周，使茎丝体发育并负向地性生长。对于向光性，将培养皿垂直放置在黑箱中，并用连续红光进行单侧照射。对于向极性，将培养皿水平放置，并从上方用线性偏振红光照射。照射后，使用带有CCD相机的体视显微镜对丝状体进行拍照，并使用图像处理软件测量弯曲角度。将数据传输到电子表格程序中，分成10°弯曲角度类别，并使用圆形直方图进行说明。
