Department of Basic Sciences, Faculty of Fisheries and Aquaculture, Kastamonu University, Kastamonu, Turkey.
Libyan Ministry of Marine Wealth, Tripoli, Libya.
J Fish Dis. 2019 Oct;42(10):1369-1381. doi: 10.1111/jfd.13061. Epub 2019 Jul 17.
Different antibiotic-based drugs are being used for the treatment of Aeromonas hydrophila infection in rainbow trout, and several studies emphasize the use of medicinal plants as immunostimulants for prophylactic measure against Aeromoniasis disease. However, therapeutic effects of aqueous methanolic extracts of tetra (Cotinus coggygria) against A. hydrophila in rainbow trout were not investigated. Four different concentrations of tetra extract (0 [control], 4, 8 and 12 mg/100 µl) and also two different positive control groups (florfenicol and doxycycline antibiotics) were administered orally using feeding needles to individual rainbow trout, Oncorhynchus mykiss of all experimental groups twice a day after intramuscular inoculation of A. hydrophila. The study period was for 10 days. On 0th, 3rd, 7th and 10th day, blood and tissues were collected from the fish and changes in humoral immune responses, haematology and immune-related gene expressions were determined. In the study, superoxide radical production was decreased generally in all experimental groups except in 12 mg tetra and florfenicol treatments compared to control (p < .05). Lysozyme activity was generally decreased (p < .05), or no differences were observed in all experimental groups compared to the control. Myeloperoxidase activity was significantly increased in florfenicol-treated fish group on 7th day (p < .05). Generally, myeloperoxidase activity showed an increase in almost all tetra-treated groups. Haematological parameters increased but were not significantly high enough in treatments. Almost all immune-related gene expressions were significantly enhanced on 3rd and 10th day of the study. Survival rate of 53.33% was found in control group. There were no significant differences in survival between control and 4 mg tetra-treated group (p > .05). All the other groups' survival rate was significantly increased compared to control. The highest survival rate was found in florfenicol group (80%). In 12 mg tetra-, doxycycline- and 8 mg tetra-treated groups, survival rate was recorded as 74.44%, 70% and 70%, respectively. Our results suggest that tetra methanolic extract is an effective therapeutic remedy against A. hydrophila infection in rainbow trout at the dose of 24 mg/32.34 g body weight/day.
不同的抗生素药物被用于治疗虹鳟鱼的嗜水气单胞菌感染，有几项研究强调使用药用植物作为预防气单胞菌病的免疫刺激剂。然而，尚未研究水甲醇提取物对虹鳟鱼嗜水气单胞菌的治疗效果。用喂食针向各组虹鳟鱼（彩虹鳟，Oncorhynchus mykiss）个体口服四种不同浓度的四（金缕梅）提取物（0[对照]、4、8 和 12mg/100µl）和两种不同的阳性对照组（氟苯尼考和强力霉素抗生素），每天两次，在肌肉内接种嗜水气单胞菌后。研究期为 10 天。在第 0、3、7 和 10 天，从鱼身上采集血液和组织，测定体液免疫反应、血液学和免疫相关基因表达的变化。在研究中，与对照组相比（p<.05），除 12mg 四和氟苯尼考处理组外，所有实验组的超氧自由基产生普遍降低。溶菌酶活性普遍降低（p<.05），或与对照组相比，所有实验组均无差异。在第 7 天，氟苯尼考处理组的髓过氧化物酶活性显著升高（p<.05）。一般来说，在几乎所有四处理组中，髓过氧化物酶活性都有所增加。血液学参数增加，但在治疗中不够显著。在研究的第 3 和第 10 天，几乎所有免疫相关基因的表达都显著增强。对照组的存活率为 53.33%。对照组与 4mg 四处理组之间的存活率无显著差异（p>.05）。与对照组相比，所有其他组的存活率均显著提高。存活率最高的是氟苯尼考组（80%）。在 12mg 四、强力霉素和 8mg 四处理组中，存活率分别为 74.44%、70%和 70%。我们的结果表明，在剂量为 24mg/32.34g 体重/天时，金缕梅甲醇提取物是治疗虹鳟嗜水气单胞菌感染的有效方法。
