Department of Biotechnology, Central University of Rajasthan, NH-8 Bandarsindri, Kishangarh Ajmer, 305817, Rajasthan, India.
Department of Chemistry, Central University of Rajasthan, NH-8, Bandarsindri, Kishangarh Ajmer, 305817, Rajasthan, India.
J Pept Sci. 2019 Aug;25(8):e3178. doi: 10.1002/psc.3178.
Pheromone peptides are an important component of bacterial quorum-sensing system. The pheromone peptide cOB1 (VAVLVLGA) of native commensal Enterococcus faecalis has also been identified as an antimicrobial peptide (AMP) and reported to kill the prototype clinical isolate strain of E. faecalis V583. In this study, the pheromone peptide cOB1 has shown to form amyloid-like structures, a characteristic which is never reported for a pheromone peptide so far. With in silico analysis, the peptide was predicted to be highly amyloidogenic. Further, under experimental conditions, cOB1 formed aggregates displaying characteristics of amyloid structures such as bathochromic shift in Congo red absorbance, enhancement in thioflavin T fluorescence, and fibrillar morphology under transmission electron microscopy. This novel property of pheromone peptide cOB1 may have some direct effects on the binding of the pheromone to the receptor cells and subsequent conjugative transfer, making this observation more important for the therapeutics, dealing with the generation of virulent and multidrug-resistant pathogenic strains.
信息素肽是细菌群体感应系统的重要组成部分。天然共生粪肠球菌的信息素肽 cOB1（VAVLVLGA）也被鉴定为一种抗菌肽（AMP），并被报道可杀死原型临床分离粪肠球菌 V583 株。在这项研究中，信息素肽 cOB1 显示出形成类似淀粉样的结构，这是迄今为止从未报道过的信息素肽的特征。通过计算机分析，该肽被预测为高度淀粉样变性。此外，在实验条件下，cOB1 形成了显示出淀粉样结构特征的聚集体，如刚果红吸收的增色效应、硫黄素 T 荧光的增强以及在透射电子显微镜下的纤维状形态。这种新型信息素肽 cOB1 的特性可能对信息素与受体细胞的结合以及随后的转导有一些直接影响，这一观察结果对治疗具有毒力和多药耐药性的致病性菌株的产生更为重要。
