Derazshamshir Ali, Aşır Süleyman, Göktürk Ilgım, Ektirici Sisem, Yılmaz Fatma, Denizli Adil
Department of Chemistry, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
Department of Materials Science and Nanotechnology Engineering, Near East University, 99138, Nicosia, North Cyprus, Mersin 10, Turkey.
J Chromatogr Sci. 2019 Aug 16;57(8):758-765. doi: 10.1093/chromsci/bmz047.
The ability to detect catecholamines (CAs) and their metabolites is vital to understand the mechanism behind the neuronal diseases. Neurochemistry aims to provide an improved pharmacological, molecular and physiological understanding of complex brain chemistries by analytical techniques. Capillary electrophoresis (CE) is one such analytical technique that enables the study of various chemical species ranging from amino acids and peptides to natural products and drugs. CE can easily adapt the changes in research focus and in recent years remains an applicable technique for investigating neuroscience and single cell neurobiology. The prepared phenylalanine-based hydrophobic monolithic column, Polymethacryloyl-L-phenylalanine [PMAPA], was used as a stationary phase in capillary electrochromatography to separate CAs that are similar in size and shape to each other including dopamine (DA) and norepinephrine (NE) via hydrophobic interactions. Separation carried out in a short period of 17 min was performed with the electrophoretic mobility of 5.54 × 10-6 m2 V-1 s-1 and 7.60 × 10-6 m2 V-1 s-1 for DA and NE, respectively, at pH 7.0, 65% acetonitrile ratio with 100 mbar applied pressure by the developed hydrophobic monolithic column without needing any extra process such as imprinting or spacer arms to immobilize ligands used in separation.
检测儿茶酚胺（CAs）及其代谢产物的能力对于理解神经疾病背后的机制至关重要。神经化学旨在通过分析技术对复杂的脑化学提供更好的药理学、分子和生理学理解。毛细管电泳（CE）就是这样一种分析技术，它能够研究从氨基酸、肽到天然产物和药物等各种化学物质。CE能够轻松适应研究重点的变化，并且近年来仍然是研究神经科学和单细胞神经生物学的适用技术。制备的基于苯丙氨酸的疏水整体柱，聚甲基丙烯酰-L-苯丙氨酸[PMAPA]，在毛细管电色谱中用作固定相，通过疏水相互作用分离大小和形状相似的儿茶酚胺，包括多巴胺（DA）和去甲肾上腺素（NE）。在pH 7.0、乙腈比例为65%、施加压力为100毫巴的条件下，使用开发的疏水整体柱，分别以5.54×10-6 m2 V-1 s-1和7.60×10-6 m2 V-1 s-1的电泳迁移率在17分钟的短时间内完成分离，无需任何额外的过程，如印迹或间隔臂来固定分离中使用的配体。
