Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.
J Chromatogr A. 2018 Nov 23;1577:66-71. doi: 10.1016/j.chroma.2018.09.046. Epub 2018 Sep 26.
A novel monolithic column based on the copolymerization of ethylene dimethacrylate and a deep eutectic solvent (DES), which composed of chlorocholine chloride and itaconic acid, was developed for capillary electrochromatography (CEC). The structure and morphology of the obtained monolithic column was characterized by Fourier transform infrared spectra and scanning electron microscopy, which showed a porous monolithic structure with good permeability. The influence of pH value and acetonitrile content on electroosmotic flow (EOF) of DES-based monolithic column was investigated. The DES-based monolithic column was used as the separation column of CEC and exhibited excellent performance for the separation of neutral compounds, phenols, toluidines, nucleosides, nucleotide bases and alkaloids. The retention mechanism might be attributed to synergistic effect of hydrophobic interaction, hydrogen bond interaction and electronic interaction. The DES-based monolithic column showed good repeatability with relative standard deviations of migration time less than 0.80%, 2.15%, 4.26% and 3.08% for intra-day, inter-day, column-to-column and batch-to-batch, respectively. Thus, organic polymer monolithic columns with DESs as functional monomers are a promising option in chromatographic separation.
一种基于乙烯基二甲酸酯和深共熔溶剂（DES）共聚的整体柱被开发用于毛细管电色谱（CEC）。该 DES 由氯化胆碱和衣康酸组成。所得整体柱的结构和形态通过傅里叶变换红外光谱和扫描电子显微镜进行了表征，其具有多孔整体结构和良好的渗透性。考察了 pH 值和乙腈含量对 DES 基整体柱电渗流（EOF）的影响。DES 基整体柱被用作 CEC 的分离柱，对中性化合物、酚类、甲苯胺、核苷、核苷酸碱基和生物碱的分离表现出优异的性能。保留机制可能归因于疏水性相互作用、氢键相互作用和电子相互作用的协同效应。DES 基整体柱表现出良好的重现性，日内、日间、柱间和批间迁移时间的相对标准偏差分别小于 0.80%、2.15%、4.26%和 3.08%。因此，以 DES 为功能单体的有机聚合物整体柱在色谱分离中是一种很有前途的选择。
