Bergman Daniel, Larsson Anders, Hansson-Hamlin Helene, Ström Holst Bodil
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
Vet Clin Pathol. 2019 Oct;48 Suppl 1:59-69. doi: 10.1111/vcp.12764. Epub 2019 Jul 18.
Canine anti-mouse antibodies are a potential source of immunoassay interference, but erroneous immunoassay results are not always easily identifiable. Anti-Müllerian hormone (AMH) is a marker for the presence of gonads in dogs, but elevated AMH concentrations in neutered dogs could also be caused by antibody interference. For other assays, a discrepant result obtained after antibody precipitation might indicate antibody interference.
We aimed to evaluate if canine anti-mouse antibodies are a source of erroneous results in the AMH assay and if antibody precipitation with polyethylene glycol (PEG) is a useful tool for detecting antibody interference in a variety of immunoassays used in the veterinary clinical laboratory.
Twenty-nine positive and 25 negative samples for anti-mouse antibodies were analyzed for AMH, canine total thyroxine (TT ), canine thyroid-stimulating hormone (TSH) and progesterone before and after treatment with PEG. Results that differed by more than four SDs from the intra-assay coefficients of variation were considered discrepant. Elevated AMH concentrations in neutered dogs with anti-mouse antibodies and no visible gonads present were considered evidence of interference.
Evidence of antibody interference was found in two samples analyzed for AMH. The presence of anti-mouse antibodies did not lead to a higher proportion of discrepant results after PEG treatment for any of the immunoassays. The overall incidence of discrepant results for healthy controls was very high (73%).
Canine anti-mouse antibodies are a source of erroneous AMH results. Antibody precipitation with PEG is not a useful tool for detecting interference caused by such antibodies.
犬抗鼠抗体是免疫测定干扰的一个潜在来源，但免疫测定的错误结果并非总是易于识别。抗苗勒管激素（AMH）是犬类性腺存在的标志物，但绝育犬中AMH浓度升高也可能由抗体干扰引起。对于其他检测，抗体沉淀后获得的不一致结果可能表明存在抗体干扰。
我们旨在评估犬抗鼠抗体是否是AMH检测中错误结果的一个来源，以及用聚乙二醇（PEG）进行抗体沉淀是否是检测兽医临床实验室中各种免疫测定中抗体干扰的有用工具。
对29份抗鼠抗体阳性样本和25份阴性样本在用PEG处理前后进行AMH、犬总甲状腺素（TT）、犬促甲状腺激素（TSH）和孕酮分析。与测定内变异系数相差超过四个标准差的结果被视为不一致。绝育犬中抗鼠抗体阳性且无可见性腺但AMH浓度升高被视为干扰的证据。
在分析的两份AMH样本中发现了抗体干扰的证据。对于任何免疫测定，抗鼠抗体的存在并未导致PEG处理后不一致结果的比例更高。健康对照中不一致结果的总体发生率非常高（73%）。
犬抗鼠抗体是AMH错误结果的一个来源。用PEG进行抗体沉淀不是检测此类抗体引起的干扰的有用工具。
