Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology , Yunnan University , Kunming , Yunnan 650091 , People's Republic of China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany , Chinese Academy of Sciences , Kunming , Yunnan 650201 , People's Republic of China.
J Agric Food Chem. 2019 Aug 14;67(32):8810-8818. doi: 10.1021/acs.jafc.9b02511. Epub 2019 Aug 1.
(L.) Osbeck is a popular fruit cultivated around the world, and its peels are sometimes used for the treatment of cough, abdominal pain, and indigestion in China. However, the peel is discarded after fruit consumption in most cases, and its chemical constituents and biological activities have not been validated before. The present study focused on evaluation of the chemical and pharmacological profile of coumarins from peels of against inflammation. The extracts and phytochemicals from peels of were prepared, and anti-inflammatory activities were carried out and , including inhibiting xylene-induced ear edema and carrageenan-induced paw edema in mice and the production of inflammatory cytokines (interleukin 1β, prostaglandin 2, and tumor-necrosis factor α) in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Results indicated that methanolic extract, ethyl acetate fraction, and four major coumarins (compounds , , , and ) inhibited swelling induced by xylene and carrageenan, separately, . Furthermore, 18 coumarins inhibited inflammatory factor secretion in macrophages primed by LPS, in which compounds , , , , showed the most pronounced change, which were comparable to dexamethasone. In summary, peel of showed an anti-inflammatory effect and coumarin compounds were responsible for regulating inflammatory mediators and cytokines, which might provide a novel nutritional strategy for inflammatory diseases.
（L.）Osbeck 是一种在世界各地广泛种植的受欢迎的水果，其果皮在中国有时用于治疗咳嗽、腹痛和消化不良。然而，在大多数情况下，果皮在食用水果后会被丢弃，其化学成分和生物活性尚未得到验证。本研究主要集中在评估 果皮中的香豆素对炎症的化学和药理学特性。从 果皮中提取和分离出的提取物和植物化学物质，并进行了抗炎活性测试，包括抑制二甲苯诱导的小鼠耳肿胀和角叉菜胶诱导的爪肿胀，以及抑制脂多糖（LPS）诱导的 RAW 264.7 细胞中炎症细胞因子（白细胞介素 1β、前列腺素 2 和肿瘤坏死因子α）的产生。结果表明，甲醇提取物、乙酸乙酯部分和四种主要香豆素（化合物 、 、 、 ）分别抑制二甲苯和角叉菜胶诱导的肿胀。此外，18 种香豆素抑制了 LPS 诱导的巨噬细胞中炎症因子的分泌，其中化合物 、 、 、 、 表现出最显著的变化，与地塞米松相当。综上所述， 果皮具有抗炎作用，香豆素类化合物是调节炎症介质和细胞因子的主要成分，这可能为炎症性疾病提供一种新的营养策略。
