Michelon William, Pirolli Mateus, Mezzari Melissa Paola, Soares Hugo Moreira, da Silva Márcio Luís Busi
Federal University of Santa Catarina, Florianópolis, SC, 88040-700, Brazil E-mail:
Baylor College of Medicine, Alkek Center for Methagenomic and Microbiome Research, One Baylor Plaza, MS BMC 385 RM 808EC, Houston, Texas 77005, USA.
Water Sci Technol. 2019 Jun;79(11):2203-2210. doi: 10.2166/wst.2019.226.
The present study assessed the carbohydrate and sugar production from Chlorella spp. biomass harvested from a field scale reactor simulating phycoremediation of swine wastewater. The microalgae biomass was mainly composed by (%): carbohydrates (41 ± 0.4), proteins (50 ± 0.4), and lipids (1.3 ± 0.5). The residual sugar present in the biomass was extracted via acid hydrolysis. Among different concentrations of sulfuric acid tested (i.e., 47, 94, 188, 281 and 563 mM), significantly higher sugar content was obtained with 188 mM (0.496 g-sugar g microalgae-DW). The concentration of sugar present in the microalgae did not differ significantly between the biomasses harvested by either centrifugation or coagulation-flocculation. Two commercially available strains of yeast (i.e., Saccharomyces cerevisiae and S. cerevisiae chardonnay) were tested for their capability to ferment sugar from lyophilized microalgae biomass. S. cerevisiae chardonnay showed a significantly faster consumption of sugar during the exponential growth phase. Both strains of yeast were capable of consuming most of the sugar added ≅ 8 g L within 24 h. Overall, the results suggest that carbohydrate-rich microalgae biomass obtained from the phycoremediation of swine wastewaters can play an important role in green design for industries seeking alternative sources of feedstock rich in sugar.
本研究评估了从模拟猪废水藻类修复的田间规模反应器收获的小球藻属生物质中碳水化合物和糖的产量。微藻生物质主要由以下成分组成（百分比）：碳水化合物（41±0.4）、蛋白质（50±0.4）和脂质（1.3±0.5）。生物质中存在的残留糖通过酸水解提取。在测试的不同硫酸浓度（即47、94、188、281和563 mM）中，188 mM时获得了显著更高的糖含量（0.496 g糖/g微藻干重）。通过离心或混凝-絮凝收获的生物质中微藻所含糖的浓度没有显著差异。测试了两种市售酵母菌株（即酿酒酵母和霞多丽酿酒酵母）从冻干微藻生物质中发酵糖的能力。霞多丽酿酒酵母在指数生长期显示出显著更快的糖消耗。两种酵母菌株都能够在24小时内消耗添加的大部分糖（约8 g/L）。总体而言，结果表明，从猪废水藻类修复中获得的富含碳水化合物的微藻生物质可以在寻求富含糖的替代原料来源的行业的绿色设计中发挥重要作用。
