胰岛素样生长因子 II mRNA 结合蛋白 3（IMP3）在霍奇金淋巴瘤诊断中的互补作用。

The complementary role of insulin-like growth factor II mRNA-binding protein 3 (IMP3) in diagnosis of Hodgkin's lymphoma.

机构信息

Faculty of Medicine, Mansoura University, Egypt.

出版信息

Ann Diagn Pathol. 2019 Oct;42:64-68. doi: 10.1016/j.anndiagpath.2019.06.006. Epub 2019 Jun 17.

DOI:10.1016/j.anndiagpath.2019.06.006

PMID:31319330

Abstract

BACKGROUND

Hodgkin's Lymphoma (HL) is a peculiar subtype of lymphoid malignancies. The etiology of HL is still unknown. Insulin-like growth factor II mRNA-binding Protein 3 (IMP3) is a member of IMP family. In HL, IMP3 is expressed in the cytoplasmic compartment of the tumor cells (in both HRS cells & LP cells) against completely negative background of non-tumor cells except for residual germinal centers.

MATERIALS & METHODS: Of 51 cases of Hodgkin's lymphoma (HL) referred to surgical pathology laboratory at oncology center, Mansoura University (OCMU), Egypt. All cases were stained for CD20, CD3, CD15, CD30 and IMP3. Regarding IMP3 antibody, The slides were then incubated with the Anti-IMP3, mouse monoclonal antibody (1:200, clone sc-365640, concentrated, California) that was used as primary antibody for IMP3 detection for 1 hour at room temperature, followed by incubation with the secondary antibody, poly HRP (horseradish peroxidase), conjugate for mouse/rabbit, for 20 minutes.

RESULTS

IMP3 showed cytoplasmic immunoreactivity in 43 (83%) cases while 8 cases were negative. The sensitivity of combined CD30 & CD15, combined CD30 & IMP3, combined CD15 & IMP3 were 96%, 98% and 94% respectively. On the other hand, the sensitivity of CD30, CD15 and IMP3 alone were 92.2%, 68.6% and 84.3% respectively. All 23 studied cases of NSCHL, all the 17 cases of MCCHL, 7 out of 8 cases of LRCHL and the only case of LDCHL had predominant T-lymphocytes in their background. On the other hand, the 2 cases of NLPHL and only case of LRCHL had predominant B-lymphocytes in their background.

CONCLUSION

IMP3 is a novel marker that is expressed in large proportion of both types of HL against nearly negative background. It has no significant increase in sensitivity in detection of the tumor cells when combined with CD30. There are insignificant relations between IMP3 expression and different clinicopathological parameters. Further studies about IMP3 on a large scale of cases are required to confirm its mechanistic role in generation of HL.

摘要

背景

霍奇金淋巴瘤（HL）是一种独特的淋巴恶性肿瘤亚型。HL 的病因仍不清楚。胰岛素样生长因子 II mRNA 结合蛋白 3（IMP3）是 IMP 家族的成员。在 HL 中，IMP3 在肿瘤细胞的细胞质部分表达（在 HRS 细胞和 LP 细胞中），而非肿瘤细胞的背景则完全呈阴性，除了残留的生发中心。

材料与方法

对来自埃及曼苏拉大学肿瘤中心（OCMU）外科病理学实验室的 51 例霍奇金淋巴瘤（HL）病例进行了研究。所有病例均进行了 CD20、CD3、CD15、CD30 和 IMP3 染色。关于 IMP3 抗体，将载玻片用 Anti-IMP3、鼠单克隆抗体（1:200，克隆 sc-365640，浓缩，加利福尼亚）孵育 1 小时，室温下作为 IMP3 检测的一抗，然后用多 HRP（辣根过氧化物酶），兔/鼠二抗孵育 20 分钟。

结果

IMP3 在 43 例（83%）病例中显示细胞质免疫反应性，8 例为阴性。联合 CD30 和 CD15、联合 CD30 和 IMP3、联合 CD15 和 IMP3 的灵敏度分别为 96%、98%和 94%。另一方面，CD30、CD15 和 IMP3 单独的灵敏度分别为 92.2%、68.6%和 84.3%。所有 23 例研究的 NSCHL、17 例 MCCHL、8 例 LRCHL 中的 7 例和仅有的 1 例 LDCHL 均具有其背景中的优势 T 淋巴细胞。另一方面，2 例 NLPHL 和仅有的 1 例 LRCHL 具有其背景中的优势 B 淋巴细胞。