过表达 Pear()基因对番茄木质素含量的影响。
Overexpression of Pear () in Tomato Affects Lignin Content.
机构信息
College of Horticulture, Qingdao Agricultural University, 700 Changcheng Road, Chengyang, Qingdao City 266109, China.
Department of Agricultural and Environmental Sciences, College of Agriculture, Tennessee State University, 3500 John Merritt Blvd, Nashville, TN 37209, USA.
出版信息
Molecules. 2019 Jul 17;24(14):2595. doi: 10.3390/molecules24142595.
was originally isolated from the 'Wangkumbae' pear ( Nakai), and it encodes for cinnamyl alcohol dehydrogenase (CAD), which is a key enzyme in the lignin biosynthesis pathway. In order to verify the function of , transgenic tomato () 'Micro-Tom' plants were generated using over-expression constructs via the agrobacterium-mediated transformation method. The results showed that the over-expression transgenic tomato plant had a strong growth vigor. Furthermore, these over-expression transgenic tomato plants contained a higher lignin content and CAD enzymatic activity in the stem, leaf and fruit pericarp tissues, and formed a greater number of vessel elements in the stem and leaf vein, compared to wild type tomato plants. This study clearly indicated that overexpressing increased the lignin deposition of transgenic tomato plants, and thus validated the function of in lignin biosynthesis.
该基因最初是从‘Wangkumbae’梨（麻梨）中分离得到的，它编码肉桂醇脱氢酶（CAD），CAD 是木质素生物合成途径中的关键酶。为了验证的功能，通过农杆菌介导的转化方法，利用过表达载体生成了转基因番茄（）‘Micro-Tom’植株。结果表明，过表达转基因番茄植株具有很强的生长活力。此外，与野生型番茄植株相比，这些过表达转基因番茄植株的茎、叶和果实果皮组织中木质素含量和 CAD 酶活性更高，在茎和叶脉中形成了更多的导管分子。本研究清楚地表明，过表达增加了转基因番茄植株的木质素沉积，从而验证了在木质素生物合成中的功能。
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