Department Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
Mount Vernon Cancer Centre, Middlesex HA6 2RN, UK.
Cells. 2019 Jul 17;8(7):732. doi: 10.3390/cells8070732.
Current diagnosis and staging of advanced epithelial ovarian cancer (aEOC) has important limitations and better biomarkers are needed. We investigate the performance of non-haematopoietic circulating cells (CCs) at the time of disease presentation and relapse. Venous blood was collected prospectively from 37 aEOC patients and 39 volunteers. CCs were evaluated using ImageStream Technology and specific antibodies to differentiate epithelial cells from haematopoetic cells. qRT-PCR from whole blood of relapsed aEOC patients was carried out for biomarker discovery. Significant numbers of CCs (CK/WT1/CD45) were identified, quantified and characterised from aEOC patients compared to volunteers. CCs are abundant in women with newly diagnosed aEOC, prior to any treatment. Evaluation of RNA from the CCs in relapsed aEOC patients (n = 5) against a 79-gene panel revealed several differentially expressed genes compared to volunteers (n = 14). Size differentiation of CCs versus CD45 haematopoietic cells was not reliable. CCs of non-haematopoetic origin are prevalent, particularly in patients with newly diagnosed aEOC. Exploiting a CC-rich population in aEOC patients offers insights into a part of the circulating microenvironment.
目前，对晚期上皮性卵巢癌（aEOC）的诊断和分期存在重要局限性，需要更好的生物标志物。我们研究了在疾病发生和复发时非造血循环细胞（CCs）的表现。前瞻性采集了 37 名 aEOC 患者和 39 名志愿者的静脉血。使用 ImageStream 技术和特定的抗体来区分上皮细胞和造血细胞，对 CCs 进行评估。对复发的 aEOC 患者的全血进行 qRT-PCR 以进行生物标志物发现。与志愿者相比，从 aEOC 患者中鉴定、定量和表征出大量的 CC（CK/WT1/CD45）。在接受任何治疗之前，新发 aEOC 女性患者中 CC 数量丰富。与志愿者（n = 14）相比，对 5 名复发的 aEOC 患者（n = 5）的 CCs 中的 RNA 进行 79 个基因表达谱分析，发现了一些差异表达的基因。CCs 与 CD45 造血细胞的大小分化不可靠。非造血来源的 CCs 普遍存在，特别是在新发 aEOC 患者中。利用 aEOC 患者富含 CC 的人群可以深入了解循环微环境的一部分。
