College of Science, University of Shanghai for Science and Technology, No. 334 Jungong Road, Shanghai, 200093, China.
Department of Allergy and Immunology, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, No. 1678 Dongfang Road, Shanghai, 200127, China.
Carbohydr Polym. 2019 Oct 15;222:115039. doi: 10.1016/j.carbpol.2019.115039. Epub 2019 Jun 28.
The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy. Chitosan (CS) solution was injected into the tumor at room temperature and automatically gelled after warming to body temperature in the present of β-glycerophosphate (β-GP). Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS/BiS-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells. More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL). Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation. Moreover, the chitosan-based hydrogel had antibacterial effects. The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.
本研究旨在设计一种具有温度敏感性的可注射水凝胶，用于安全高效的体内结肠癌热疗和化疗。壳聚糖（CS）溶液在室温下注入肿瘤，在β-甘油磷酸（β-GP）存在下升温至体温时自动凝胶化。通过将光热材料 MoS/BiS-PEG（MBP）纳米片和药物分子阿霉素（DOX）溶解到水凝胶中，实现了局部肿瘤光热和化疗的联合治疗，水凝胶系统可以包裹 DOX 和 MBP 纳米片，防止它们进入血液循环，损伤正常组织和细胞。更重要的是，在低 MBP 浓度（0.5mg/mL）下，CS/MBP/DOX（CMD）水凝胶在第一和第二近红外光（NIR I 和 NIR II）生物窗口分别表现出 22.18%和 31.42%的光热效率。此外，由于凝胶温度可以通过近红外激光照射来控制，因此可以控制 CMD 水凝胶中 DOX 的释放。此外，壳聚糖基水凝胶具有抗菌作用。由于 NIR I 和 NIR II 的不同穿透深度，预计设计的复合水凝胶将作为高效治疗肿瘤的平台。
