Chemical and Biochemical Processing Division, ICAR-Central Institute for Research on Cotton Technology, Mumbai 400019, India.
Chemical and Biochemical Processing Division, ICAR-Central Institute for Research on Cotton Technology, Mumbai 400019, India.
Carbohydr Polym. 2019 Oct 15;222:115021. doi: 10.1016/j.carbpol.2019.115021. Epub 2019 Jun 24.
We reported the preparation of antibacterial corn starch film (57% reduction in bacterial count) with enhanced tensile strength (69%) by incorporating immobilized bacteriocin. Whisker shaped crystalline nanocellulose (CNC, length 71.2 ± 20.7 nm and width 27.8 ± 11.2 nm) was prepared from cotton linters by bio-mechanical process, having the degree of polymerization 250. Bacteriocins extracted from broth cultures of P. acidilactici and E. faecium were immobilized on the surface of CNC and used to reinforce the starch film. The biodegradability of reinforced films was affected due to the use of bacteriocin in fillers. Surface morphology and roughness of reinforced films were studied by SEM and AFM. In an ambient environment, the films incorporated with bacteriocin immobilized CNC stayed fresh for 28 days while that of bacteriocin alone had fungal degradation in 14 days. This supports the requirement of CNC immobilization for better stability of bacteriocin on storage.
我们报道了一种抗菌玉米淀粉膜的制备方法，通过固定抑菌素来提高其拉伸强度（提高 69%），同时降低细菌数量（降低 57%）。晶须状纳米纤维素（CNC，长度 71.2±20.7nm，宽度 27.8±11.2nm）由棉绒通过生物力学过程制备，聚合度为 250。从发酵液中提取的抑菌素被固定在 CNC 的表面，并用于增强淀粉膜。由于在填充剂中使用了抑菌素，增强膜的生物降解性受到了影响。通过 SEM 和 AFM 研究了增强膜的表面形貌和粗糙度。在环境条件下，含有固定化抑菌素的 CNC 的薄膜在 28 天内保持新鲜，而单独含有抑菌素的薄膜在 14 天内就发生了真菌降解。这支持了在储存时需要将 CNC 固定化以提高抑菌素稳定性的要求。
