ICAR-Central Institute for Research on Cotton Technology (ICAR-CIRCOT), Matunga, Mumbai 400019, India.
ICAR-Central Institute for Research on Cotton Technology (ICAR-CIRCOT), Matunga, Mumbai 400019, India.
Carbohydr Polym. 2019 Apr 1;209:172-180. doi: 10.1016/j.carbpol.2019.01.027. Epub 2019 Jan 9.
Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min). The bacteriocin was sensitive to protease treatment and most active in acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison. To enhance stability for diversified applications, the bacteriocin was immobilized by physical adsorption onto cellulose nanocrystals (CNC) extracted from cotton linters. The bacteriocin immobilization yield was 64.91% for P. acidilactici and 53.63% for E. faecium. The bacteriocin immobilized CNC was characterized by DLS particle sizing, FTIR and AFM to evaluate size distribution, chemical nature and surface morphology. The bacteriocins immobilized on CNC showed 50% increase in stability in terms of antibacterial activity. The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.
从豇豆中分离出的屎肠球菌（E. faecium）产生的细菌素能抑制革兰氏阳性菌和革兰氏阴性菌，且热稳定性更好（100°C 处理 30 分钟）。该细菌素对蛋白酶处理敏感，在酸性 pH 值下活性最高。使用副干酪乳杆菌产生的细菌素来进行比较。为了提高稳定性以应用于多样化的领域，将细菌素通过物理吸附固定到从棉绒中提取的纤维素纳米晶体（CNC）上。副干酪乳杆菌和屎肠球菌的细菌素固定化收率分别为 64.91%和 53.63%。通过 DLS 粒径分析、FTIR 和 AFM 对固定化 CNC 的细菌素进行了表征，以评估其粒径分布、化学性质和表面形态。固定在 CNC 上的细菌素在抗菌活性方面的稳定性提高了 50%。在 CNC 酶合成的基础上，结合细菌素的物理吸附固定化方法，使其成为一种用于食品包装和生物复合材料应用的生产抗菌纳米填料的有效体系。
