School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China; Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei, 230009, China.
School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China; Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei, 230009, China.
Carbohydr Polym. 2019 Oct 15;222:115028. doi: 10.1016/j.carbpol.2019.115028. Epub 2019 Jun 26.
A homogenous polysaccharide (GXG) from Dendrobium huoshanense with stable digestive behavior and effective immunoregulatory function was employed to explore its underlying molecular basis regulating intestinal mucosal immune response from the view of interaction between GXG and intestinal epithelial cells (IECs). Using in vitro established co-culture system consisting of IECs and lamina propria cells (LPCs), we found the immune response of LPCs could be effectively regulated by GXG-stimulated IECs, and three cytokines including IL-6, MCP-1 and CINC-1 produced from GXG-stimulated IECs were the main factors involved in modulating immune response of LPCs. Toll-like receptor 4 (TLR4) was identified as an essential receptor for IECs to directly bind GXG. Receptor intervention experiments demonstrated that TLR4 mediated GXG-induced activation of IECs, which further induces immunomodulating effects on LPCs. These results suggest that GXG could modulate the immune response in LPCs by the direct interaction with IECs via TLR4.
一种来自霍山石斛的均一多糖（GXG）具有稳定的消化行为和有效的免疫调节功能，从 GXG 与肠上皮细胞（IECs）相互作用的角度出发，我们利用体外建立的包含 IECs 和固有层细胞（LPCs）的共培养系统，来探索其调节肠道黏膜免疫应答的潜在分子基础。研究发现，GXG 刺激的 IECs 可有效调节 LPCs 的免疫应答，且来自 GXG 刺激的 IECs 的三种细胞因子（IL-6、MCP-1 和 CINC-1）是调节 LPCs 免疫应答的主要因素。Toll 样受体 4（TLR4）被鉴定为 IECs 直接结合 GXG 的必需受体。受体干预实验表明，TLR4 介导了 GXG 诱导的 IECs 激活，进而对 LPCs 产生免疫调节作用。这些结果表明，GXG 可以通过 TLR4 与 IECs 的直接相互作用来调节 LPCs 的免疫应答。
