埃塞俄比亚西北部贡德尔镇食品从业人员肠道细菌的流行情况及其对抗菌药物的敏感性模式。
Prevalence of enteric bacteria and their antimicrobial susceptibility patterns among food handlers in Gondar town, Northwest Ethiopia.
机构信息
1Department of Laboratory, University of Gondar Comprehensive Specialized Hospital, Gondar, Ethiopia.
2Department of Medical Microbiology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, P.O.Box:196, Gondar, Ethiopia.
出版信息
Antimicrob Resist Infect Control. 2019 Jul 8;8:111. doi: 10.1186/s13756-019-0566-7. eCollection 2019.
BACKGROUND
Enteric bacterial pathogens are the major causes of food-borne gastroenteritis in humans and remain important public health problems worldwide. The emergence of antimicrobial resistance is a global concern, particularly in developing countries. The aim of this study was to determine the prevalence of enteric bacteria pathogens and their antimicrobial susceptibility patterns among food handlers in Gondar town, Northwest Ethiopia.
METHODS
A cross-sectional study was conducted from February 4 to April 16, 2018. A total of 257 food handlers were selected using a multistage sampling technique. Data on socio-demographic characteristics were collected using a structured questionnaire. Stool samples were collected and inoculated into appropriate media. Enteric bacterial pathogens were identified using standard microbiological methods. Antimicrobial susceptibility tests were performed using the disk diffusion technique as per the standard Kirby-Bauer method. Data were entered and analyzed using SPSS version 20 software.
RESULTS
The overall prevalence of enteric bacteria was 34/257 (13. 2%, [95% CI, 8.9-17.5%]). species was the leading isolate that accounted for 26/257 (10.1%) followed by Enterohemorrhagic (EHEC) O157: H7 5/257 (1.9%) and species 3/257 (1.2%). spp. was susceptible to ciprofloxacin 26 (100%), ceftriaxone 25 (96.1%), chloramphenicol 24 (92.3%), nalidixic acid 24 (92.3%), and gentamicin 20 (76.9%). O157: H7 and spp. showed the maximum (100%) susceptibility results to ceftriaxone, chloramphenicol, ciprofloxacin, and gentamicin. The overall prevalence of Multidrug resistance (MDR) in the current study was 14/34 (41.2%).
CONCLUSION
Our study showed high prevalence of enteric bacterial pathogens among food handlers. All isolates were susceptible to ciprofloxacin. However, a substential number of isolates were resistant to commonly prescribed antibiotics and the prevalence of MDR was high.
背景
肠细菌病原体是人类食源性肠胃炎的主要病因，仍是全球范围内重要的公共卫生问题。抗生素耐药性的出现是一个全球性关注的问题，尤其是在发展中国家。本研究旨在确定在埃塞俄比亚西北部贡德尔镇的食品从业人员中肠细菌病原体的流行情况及其对抗生素的敏感性模式。
方法
本横断面研究于 2018 年 2 月 4 日至 4 月 16 日进行。采用多阶段抽样技术选择了 257 名食品从业人员。使用结构化问卷收集社会人口统计学特征数据。采集粪便样本并接种于适当的培养基中。采用标准微生物学方法鉴定肠细菌病原体。采用纸片扩散法按标准 Kirby-Bauer 法进行抗生素敏感性试验。使用 SPSS 版本 20 软件录入和分析数据。
结果
肠细菌的总流行率为 34/257（13.2%，[95%置信区间，8.9-17.5%]）。 是主要的分离株，占 26/257（10.1%），其次是肠出血性 （EHEC）O157：H7 5/257（1.9%）和 3/257（1.2%）。 spp.对环丙沙星 26（100%）、头孢曲松 25（96.1%）、氯霉素 24（92.3%）、萘啶酸 24（92.3%）和庆大霉素 20（76.9%）敏感。O157：H7 和 spp.对头孢曲松、氯霉素、环丙沙星和庆大霉素的敏感性最高（100%）。本研究中多药耐药（MDR）的总流行率为 14/34（41.2%）。
结论
我们的研究表明，食品从业人员中肠细菌病原体的流行率较高。所有分离株均对环丙沙星敏感。然而，相当数量的分离株对常用抗生素耐药，且 MDR 的流行率较高。
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