富含锌的益生菌增强了在高环境温度下饲养的Wistar大鼠的生长性能、抗氧化状态、免疫功能、基因表达和形态特征。
Zinc-enriched probiotics enhanced growth performance, antioxidant status, immune function, gene expression, and morphological characteristics of Wistar rats raised under high ambient temperature.
作者信息
Mohammad Malyar Rahmani, Li Hu, Enayatullah Hamdard, Hou Lili, Ahmad Farid Rawan, Liu Dandan, Akhter Bhat Javaid, Miao Jinfeng, Gan Fang, Huang Kehe, Chen Xingxiang
机构信息
1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095 China.
2Faculty of Veterinary Science, Nangarhar University, Jalalabad, Nangarhar Province Afghanistan.
出版信息
3 Biotech. 2019 Aug;9(8):291. doi: 10.1007/s13205-019-1819-0. Epub 2019 Jul 2.
The present study was conducted to evaluate the effects of zinc-enriched probiotics (ZnP) on growth performance, antioxidant status, immune function, related gene expression, and morphological characteristics of Wistar rats raised under high heat stress condition during summer. 36, 6-week-old male Wistar rats were randomly divided into three groups; fed with basal diet (control), basal diet with probiotics (P), and basal diet with zinc-enriched probiotics supplementation (ZnP, 100 mg/L), for 40 consecutive days. Blood samples were collected through intracardiac method on the last day of experiment and tissues were collected from liver, heart, and kidneys. The results revealed that both P and ZnP significantly (< 0.05) enhanced growth performance. However, ZnP remarkably increased glutathione content, glutathione peroxidase, and superoxide dismutase activities but reduced malondialdehyde level in serum of the Wistar rats. The concentration of IL-2, IL-6, and IFN-γ was significantly (< 0.05) increased with treatments of P and ZnP compared to control group while IL-10 was significantly (< 0.05) decreased. Additionally, the expression of SOD1, SOD2, MT1, and MT2 genes was significantly (< 0.05) up-regulated with the treatment of ZnP, but Hsp90 and Hsp70 heat shock genes were significantly (< 0.05) down-regulated with the treatment of P and ZnP, respectively. Hematoxylin and Eosin staining showed that both P and ZnP supplementation treatments induced changes in villus height and intestinal wall thickness. In conclusion, zinc-enriched probiotics supplementation can improve the growth performance of Wistar rats under high ambient temperature through enhancing antioxidant status, immune function, related genes expression, and intestinal morphological characteristics. This product may serves as a potential nutritive supplement for Wistar rats under high heat stress conditions.
本研究旨在评估富锌益生菌（ZnP）对夏季高温应激条件下饲养的Wistar大鼠生长性能、抗氧化状态、免疫功能、相关基因表达及形态特征的影响。将36只6周龄雄性Wistar大鼠随机分为三组，分别连续40天饲喂基础日粮（对照组）、添加益生菌的基础日粮（P组）和添加富锌益生菌的基础日粮（ZnP组，100 mg/L）。实验最后一天通过心脏采血采集血样，并从肝脏、心脏和肾脏采集组织。结果显示，P组和ZnP组均显著（<0.05）提高了生长性能。然而，ZnP显著增加了Wistar大鼠血清中的谷胱甘肽含量、谷胱甘肽过氧化物酶和超氧化物歧化酶活性，但降低了丙二醛水平。与对照组相比，P组和ZnP组处理后白细胞介素-2、白细胞介素-6和干扰素-γ的浓度显著（<0.05）升高，而白细胞介素-10显著（<0.05）降低。此外，ZnP处理显著（<0.05）上调了超氧化物歧化酶1、超氧化物歧化酶2、金属硫蛋白1和金属硫蛋白2基因的表达，但P组和ZnP组处理分别显著（<0.05）下调了热休克蛋白90和热休克蛋白70热休克基因的表达。苏木精和伊红染色显示，P组和ZnP组补充处理均引起绒毛高度和肠壁厚度的变化。总之，补充富锌益生菌可通过增强抗氧化状态、免疫功能、相关基因表达和肠道形态特征来提高高温环境下Wistar大鼠的生长性能。该产品可能作为高温应激条件下Wistar大鼠的潜在营养补充剂。
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