Key Laboratory of Meat Processing and Quality Control, MOE, Key Laboratory of Meat Processing, MOA, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, Nanjing Agricultural University, Nanjing, P.R. China.
Guangxi Vocational College of Technology and Business, Nanning, Guangxi, P.R. China.
Mol Nutr Food Res. 2018 Feb;62(4). doi: 10.1002/mnfr.201700661. Epub 2018 Jan 29.
Dietary fats have been shown to affect gut microbiota composition and aging gene expression of middle-aged rats at a normal dose, but little is known about such an effect on gut barrier. In this study, the changes in colonic Muc2 expression are investigated and the underlying mechanism is also proposed.
36 middle-aged Sprague-Dawley rats are assigned to one of the diets containing soybean oil, lard, or fish oil (4%). The rats are fed for 5 weeks and then goblet cells, Muc2 expression, and inflammatory cytokines in the colon are measured. Proteome analysis is performed. Compared with the lard and soybean oil diet groups, intake of fish oil decreases the number of goblet cells, and inhibits Muc2 and TLRs expression in the colon of middle-aged rats, which would impair mucus barrier. Several key enzymes involved in glycosylation process, including Agr2, Gale, Gne, Pmm2, Pdxdc1, Plch1, Pfkp, Cmpk1, and Rexo2, show the lowest abundance in the fish oil diet group.
Intake of fish oil at a normal dose downregulates colonic Muc2 expression. This negative effect of fish oil may involve the suppression of mucin glycosylation process.
膳食脂肪已被证明会影响正常剂量下中年大鼠的肠道微生物组成和衰老基因表达，但对于其对肠道屏障的影响知之甚少。在本研究中，我们研究了结肠 Muc2 表达的变化，并提出了其潜在机制。
将 36 只中年 Sprague-Dawley 大鼠分为大豆油组、猪油组或鱼油组（4%）中的一组。大鼠喂养 5 周后，测量结肠中的杯状细胞、Muc2 表达和炎症细胞因子。进行蛋白质组分析。与猪油和大豆油饮食组相比，摄入鱼油会减少杯状细胞的数量，并抑制中年大鼠结肠中 Muc2 和 TLRs 的表达，从而损害黏液屏障。糖苷化过程中涉及的几个关键酶，包括 Agr2、Gale、Gne、Pmm2、Pdxdc1、Plch1、Pfkp、Cmpk1 和 Rexo2，在鱼油饮食组中的丰度最低。
正常剂量摄入鱼油会下调结肠 Muc2 的表达。鱼油的这种负面影响可能涉及粘蛋白糖基化过程的抑制。
