Department of Biology, Baylor University, Waco, TX, USA.
FEBS Lett. 2019 Nov;593(22):3228-3238. doi: 10.1002/1873-3468.13545. Epub 2019 Jul 30.
The nonsense-mediated mRNA decay (NMD) pathway regulates mRNAs that aberrantly terminate translation. This includes aberrant mRNAs and functional natural mRNAs. Natural mRNA degradation by NMD is triggered by mRNA features and environmental cues. Saccharomyces cerevisiae encodes multiple proteins with ferric and cupric reductase activity. Here, we examined the regulation by NMD of two mRNAs, FRE1 and FRE2, encoding ferric and cupric reductases in S. cerevisiae. We found that FRE2 mRNAs are regulated by NMD under noninducing conditions and that the FRE2 3'-UTR contributes to the degradation of the mRNAs by NMD. Conversely, FRE1 mRNAs are not regulated by NMD under comparable conditions. These findings suggest that regulation of functionally related mRNAs by NMD can be differential and conditional.
无意义介导的 mRNA 降解 (NMD) 途径调节异常终止翻译的 mRNA。这包括异常的 mRNA 和功能正常的天然 mRNA。NMD 通过 mRNA 特征和环境线索触发天然 mRNA 的降解。酿酒酵母编码多种具有铁还原酶和铜还原酶活性的蛋白质。在这里，我们研究了 NMD 对编码酿酒酵母中铁还原酶和铜还原酶的两个 mRNA（FRE1 和 FRE2）的调节。我们发现，在非诱导条件下，FRE2 mRNAs 受 NMD 调节，并且 FRE2 3'-UTR 有助于 NMD 降解这些 mRNAs。相反，在类似条件下，FRE1 mRNAs 不受 NMD 调节。这些发现表明，NMD 对功能相关 mRNAs 的调节可以是差异的和有条件的。
