酵母中 ferric 和 cupric reductase mRNAs FRE1 和 FRE2 的无意义介导的 mRNA 衰变。
Nonsense-mediated mRNA decay of the ferric and cupric reductase mRNAs FRE1 and FRE2 in Saccharomyces cerevisiae.
机构信息
Department of Biology, Baylor University, Waco, TX, USA.
出版信息
FEBS Lett. 2019 Nov;593(22):3228-3238. doi: 10.1002/1873-3468.13545. Epub 2019 Jul 30.
Abstract
The nonsense-mediated mRNA decay (NMD) pathway regulates mRNAs that aberrantly terminate translation. This includes aberrant mRNAs and functional natural mRNAs. Natural mRNA degradation by NMD is triggered by mRNA features and environmental cues. Saccharomyces cerevisiae encodes multiple proteins with ferric and cupric reductase activity. Here, we examined the regulation by NMD of two mRNAs, FRE1 and FRE2, encoding ferric and cupric reductases in S. cerevisiae. We found that FRE2 mRNAs are regulated by NMD under noninducing conditions and that the FRE2 3'-UTR contributes to the degradation of the mRNAs by NMD. Conversely, FRE1 mRNAs are not regulated by NMD under comparable conditions. These findings suggest that regulation of functionally related mRNAs by NMD can be differential and conditional.
摘要
无意义介导的 mRNA 降解 (NMD) 途径调节异常终止翻译的 mRNA。这包括异常的 mRNA 和功能正常的天然 mRNA。NMD 通过 mRNA 特征和环境线索触发天然 mRNA 的降解。酿酒酵母编码多种具有铁还原酶和铜还原酶活性的蛋白质。在这里，我们研究了 NMD 对编码酿酒酵母中铁还原酶和铜还原酶的两个 mRNA（FRE1 和 FRE2）的调节。我们发现，在非诱导条件下，FRE2 mRNAs 受 NMD 调节，并且 FRE2 3'-UTR 有助于 NMD 降解这些 mRNAs。相反，在类似条件下，FRE1 mRNAs 不受 NMD 调节。这些发现表明，NMD 对功能相关 mRNAs 的调节可以是差异的和有条件的。
相似文献
1
Nonsense-mediated mRNA decay of the ferric and cupric reductase mRNAs FRE1 and FRE2 in Saccharomyces cerevisiae.酵母中 ferric 和 cupric reductase mRNAs FRE1 和 FRE2 的无意义介导的 mRNA 衰变。
FEBS Lett. 2019 Nov;593(22):3228-3238. doi: 10.1002/1873-3468.13545. Epub 2019 Jul 30.
2
mRNAs involved in copper homeostasis are regulated by the nonsense-mediated mRNA decay pathway depending on environmental conditions.参与铜稳态的信使核糖核酸（mRNA）根据环境条件，由无义介导的mRNA降解途径调控。
Fungal Genet Biol. 2016 Jan;86:81-90. doi: 10.1016/j.fgb.2015.12.011. Epub 2015 Dec 19.
3
The nonsense-mediated mRNA decay (NMD) pathway differentially regulates COX17, COX19 and COX23 mRNAs.无义介导的mRNA降解（NMD）途径对COX17、COX19和COX23 mRNA具有差异调节作用。
Curr Genet. 2019 Apr;65(2):507-521. doi: 10.1007/s00294-018-0892-y. Epub 2018 Oct 13.
4
Nonsense-mediated mRNA decay of metal-binding activator MAC1 is dependent on copper levels and 3'-UTR length in Saccharomyces cerevisiae.金属结合激活因子 MAC1 的无意义介导的 mRNA 降解依赖于酿酒酵母中的铜水平和 3'-UTR 长度。
Curr Genet. 2024 May 6;70(1):5. doi: 10.1007/s00294-024-01291-9.
5
Regulation of CTR2 mRNA by the nonsense-mediated mRNA decay pathway.无义介导的mRNA降解途径对CTR2 mRNA的调控
Biochim Biophys Acta. 2014 Nov;1839(11):1283-94. doi: 10.1016/j.bbagrm.2014.09.011. Epub 2014 Sep 23.
6
Copper tolerance of Saccharomyces cerevisiae nonsense-mediated mRNA decay mutants.酿酒酵母无义介导的 mRNA 降解突变体对铜的耐受性。
Curr Genet. 2011 Dec;57(6):421-30. doi: 10.1007/s00294-011-0356-0. Epub 2011 Sep 15.
7
Long 3'-UTRs target wild-type mRNAs for nonsense-mediated mRNA decay in Saccharomyces cerevisiae.在酿酒酵母中，长3'非翻译区靶向野生型信使核糖核酸进行无义介导的信使核糖核酸降解。
Nucleic Acids Res. 2009 May;37(9):2771-8. doi: 10.1093/nar/gkp146. Epub 2009 Mar 6.
8
Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae.酿酒酵母中FRE1和FRE2同源物的金属调控
J Biol Chem. 1998 Sep 11;273(37):23716-21. doi: 10.1074/jbc.273.37.23716.
9
Physiological basis of copper tolerance of Saccharomyces cerevisiae nonsense-mediated mRNA decay mutants.酵母细胞非依赖意义mRNA 降解突变体耐铜的生理学基础。
Yeast. 2013 May;30(5):179-90. doi: 10.1002/yea.2950. Epub 2013 Apr 12.
10
Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes.酿酒酵母Fre1p/Fre2p铁/铜还原酶相关基因的调控表达。
Yeast. 1999 May;15(7):573-84. doi: 10.1002/(SICI)1097-0061(199905)15:7<573::AID-YEA404>3.0.CO;2-7.
引用本文的文献
1
Iron Homeostatic Regulation in Saccharomyces cerevisiae: Introduction to a Computational Modeling Method.酵母铁稳态调节：计算建模方法简介。
Methods Mol Biol. 2024;2839:3-29. doi: 10.1007/978-1-0716-4043-2_1.
2
Nonsense-mediated mRNA decay and metal ion homeostasis and detoxification in Saccharomyces cerevisiae.酿酒酵母中非编码 RNA 介导的 mRNA 降解与金属离子内稳和解毒。
Biometals. 2022 Dec;35(6):1145-1156. doi: 10.1007/s10534-022-00450-0. Epub 2022 Oct 18.
3
Variation of the response to metal ions and nonsense-mediated mRNA decay across different Saccharomyces cerevisiae genetic backgrounds.不同酿酒酵母遗传背景下对金属离子的反应和无义介导的 mRNA 降解的变化。
Yeast. 2021 Sep;38(9):507-520. doi: 10.1002/yea.3565. Epub 2021 May 21.
本文引用的文献
1
The nonsense-mediated mRNA decay (NMD) pathway differentially regulates COX17, COX19 and COX23 mRNAs.无义介导的mRNA降解（NMD）途径对COX17、COX19和COX23 mRNA具有差异调节作用。
Curr Genet. 2019 Apr;65(2):507-521. doi: 10.1007/s00294-018-0892-y. Epub 2018 Oct 13.
2
Transcript Buffering: A Balancing Act between mRNA Synthesis and mRNA Degradation.转录本缓冲：mRNA 合成与 mRNA 降解之间的平衡。
Mol Cell. 2018 Oct 4;72(1):10-17. doi: 10.1016/j.molcel.2018.08.023.
3
NMD monitors translational fidelity 24/7.无义介导的mRNA降解（NMD）全天候监测翻译保真度。
Curr Genet. 2017 Dec;63(6):1007-1010. doi: 10.1007/s00294-017-0709-4. Epub 2017 May 23.
4
Proteomic Analysis Reveals Branch-specific Regulation of the Unfolded Protein Response by Nonsense-mediated mRNA Decay.蛋白质组学分析揭示了无义介导的mRNA衰变对未折叠蛋白反应的分支特异性调控。
Mol Cell Proteomics. 2016 May;15(5):1584-97. doi: 10.1074/mcp.M115.054056. Epub 2016 Feb 20.
5
mRNAs involved in copper homeostasis are regulated by the nonsense-mediated mRNA decay pathway depending on environmental conditions.参与铜稳态的信使核糖核酸（mRNA）根据环境条件，由无义介导的mRNA降解途径调控。
Fungal Genet Biol. 2016 Jan;86:81-90. doi: 10.1016/j.fgb.2015.12.011. Epub 2015 Dec 19.
6
Measurement of mRNA decay rates in Saccharomyces cerevisiae using rpb1-1 strains.使用rpb1-1菌株测量酿酒酵母中的mRNA衰减率。
J Vis Exp. 2014 Dec 13(94):52240. doi: 10.3791/52240.
7
Regulation of CTR2 mRNA by the nonsense-mediated mRNA decay pathway.无义介导的mRNA降解途径对CTR2 mRNA的调控
Biochim Biophys Acta. 2014 Nov;1839(11):1283-94. doi: 10.1016/j.bbagrm.2014.09.011. Epub 2014 Sep 23.
8
Regulation of natural mRNAs by the nonsense-mediated mRNA decay pathway.无义介导的mRNA降解途径对天然mRNA的调控。
Eukaryot Cell. 2014 Sep;13(9):1126-35. doi: 10.1128/EC.00090-14. Epub 2014 Jul 18.
9
Copper tolerance of Saccharomyces cerevisiae nonsense-mediated mRNA decay mutants.酿酒酵母无义介导的 mRNA 降解突变体对铜的耐受性。
Curr Genet. 2011 Dec;57(6):421-30. doi: 10.1007/s00294-011-0356-0. Epub 2011 Sep 15.
10
Endogenous ribosomal frameshift signals operate as mRNA destabilizing elements through at least two molecular pathways in yeast.内源性核糖体移码信号通过至少两种分子途径在酵母中作为 mRNA 不稳定元件发挥作用。
Nucleic Acids Res. 2011 Apr;39(7):2799-808. doi: 10.1093/nar/gkq1220. Epub 2010 Nov 24.
Zotero 插件