School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea.
Developmental Epigenetics Laboratory, Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA.
Int J Mol Sci. 2023 Jan 10;24(2):1385. doi: 10.3390/ijms24021385.
Ligand of Numb-protein X 2 (LNX2) is an E3 ubiquitin ligase that is known to regulate Notch signaling by participating in NUMB protein degradation. Notch signaling is important for differentiation and proliferation in mammals, and plays a significant role in blastocyst formation during early embryonic development. In this study, we investigated in mouse preimplantation embryos. Expression analysis showed that is expressed in oocytes and preimplantation embryos. -knockdown embryos normally progress to the morula stage, but the majority of them do not develop into normal blastocysts. Transcript analysis revealed that the expression levels of genes critical for cell lineage specification, including octamer-binding transcription factor 4 (), are increased in knockdown embryos. Furthermore, the expression levels of Notch and Hippo signaling-related genes are also increased by knockdown. Collectively, our results show that is important for blastocyst formation in mice, suggest that this may act via lineage specification of inner cell mass, and further show that may be involved in transcriptionally regulating various genes implicated in early embryonic development.
神经嵴衍生分泌因子 2 配体（LNX2）是一种 E3 泛素连接酶，已知通过参与 NUMB 蛋白降解来调节 Notch 信号通路。Notch 信号通路在哺乳动物的分化和增殖中起重要作用，并在早期胚胎发育中的囊胚形成中发挥重要作用。在这项研究中，我们研究了其在小鼠植入前胚胎中的作用。表达分析表明， 在卵母细胞和植入前胚胎中均有表达。 敲低胚胎正常发育到桑葚胚阶段，但大多数胚胎不能发育成正常的囊胚。转录分析显示，参与细胞谱系特化的关键基因，包括八聚体结合转录因子 4（）的表达水平在 敲低胚胎中增加。此外，Notch 和 Hippo 信号通路相关基因的表达水平也因 敲低而增加。综上所述，我们的研究结果表明， 在小鼠囊胚形成中具有重要作用，提示其可能通过内细胞团的谱系特化起作用，进一步表明其可能参与转录调控与早期胚胎发育相关的各种基因。
