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[64kD雄激素依赖性附睾前白蛋白亲和位点在公羊精子头部的定位]

[Localization, on the head of ram spermatozoa, of affinity sites for the 64kD androgen dependent epididymal prealbumin].

作者信息

Fournier-Delpech S, Venien A M, Pisselet C, Courot M

机构信息

I.N.R.A., Centre de Recherches de Tours, Nouzilly.

出版信息

C R Acad Sci III. 1988;306(11):333-8.

PMID:3132311
Abstract

The epididymis of the ram synthesizes under androgenic control of specific 64 kD protein. The purified protein appeared as a single band in SDS polyacrylamide gel electrophoresis. It was labelled and an antiserum was prepared in mice. Results showed localization of receptors sites for 64 kD on the periacrosomal plasma membrane of testicular spermatozoa. The protein itself was found on the periacrosomal area of both epididymal and ejaculated spermatozoa.

摘要

公羊的附睾在雄激素的控制下合成一种特定的64kD蛋白质。纯化后的蛋白质在SDS聚丙烯酰胺凝胶电泳中呈现为单一条带。对其进行标记并在小鼠体内制备抗血清。结果显示,64kD受体位点定位于睾丸精子顶体周围的质膜上。该蛋白质本身则在附睾精子和射出精子的顶体周围区域被发现。

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C R Acad Sci III. 1988;306(11):333-8.
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Albumin is synthesized in epididymis and aggregates in a high molecular mass glycoprotein complex involved in sperm-egg fertilization.白蛋白在附睾中合成,并聚集在一种参与精卵受精的高分子量糖蛋白复合物中。
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