苍艾挥发油改善慢性不可预知温和应激诱导大鼠的抑郁样行为及调控脑内 DA 和 5-HT 代谢。
Cang-ai volatile oil improves depressive-like behaviors and regulates DA and 5-HT metabolism in the brains of CUMS-induced rats.
机构信息
Department of Clinical Medicine, Yunnan University of Chinese Medicine, Kunming, 650500, China.
Department of Integrative Medicine on Pediatrics, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China.
出版信息
J Ethnopharmacol. 2019 Nov 15;244:112088. doi: 10.1016/j.jep.2019.112088. Epub 2019 Jul 16.
ETHNOPHARMACOLOGICAL RELEVANCE
Cang-ai volatile oil (CAVO) is a traditional Chinese medicine (TCM) inhalational preparation for the treatment of some depressive and emotive disorders.
AIM OF THE STUDY
This research aimed to evaluate the efficiency and possible mechanism of intranasal CAVO administration on depression in chronic unpredictable mild stress (CUMS)-induced rats compared to lavender volatile oil (LVO) treatment after CUMS exposure and bilateral olfactory bulb impairment (OBI) in rats.
MATERIALS AND METHODS
Forty depressive-like model rats induced by CUMS were evaluated by the forced swim test (FST), open field test (OFT), and sucrose preference test (SPT). The model rats were divided into five groups: CUMS (n = 8), CAVOh + CUMS (n = 8), CAVOl + CUMS (n = 8), LVO + CUMS (n = 8), and OBI + CAVO + CUMS (n = 8). The CUMS-induced rats were treated for a period of 4 weeks. The other healthy rats were regarded as the control (CTR, n = 8) subjects. The levels of serotonin (5-HT) and dopamine (DA) and their respective metabolites homovanillic acid (HVA) and 5-hydroxyindol acetic acid (5-HIAA) were measured in brain tissue homogenates of CUMS-induced rats using enzyme-linked immunosorbent assay (ELISA).
RESULTS
CAVO ameliorated depressive-like behaviors (p < 0.05). The levels of DA in the CUMS group were lower than those in the CTR and CAVOh groups (**p < 0.01 and *p < 0.05). The levels of HVA were lower in the CUMS group than in the CTR, LVO, OBI + CAVOh and CAVOh groups (**p < 0.01 and *p < 0.05) and lower in the OBI + CAVOh group than in the CAVOh group (**p < 0.01). The levels of 5-HT in the CUMS group were lower than those in the CTR and CAVOh groups (**p < 0.01). The levels of 5-HIAA were lower in the CUMS and OBI + CAVOh groups than in the CTR, LVO and CAVOh groups (**p < 0.01).
CONCLUSIONS
CAVO can improve depressive-like behaviors concomitant with the regulation of DA and 5-HT metabolism in the brains of CUMS-induced rats.
民族药理学相关性
苍艾挥发油（CAVO）是一种传统的中药（TCM）吸入制剂，用于治疗某些抑郁和情感障碍。
目的
本研究旨在评估鼻腔给予苍艾挥发油（CAVO）对慢性不可预测轻度应激（CUMS）诱导的大鼠抑郁的疗效，并与薰衣草挥发油（LVO）治疗后进行比较，同时评估双侧嗅球损伤（OBI）对大鼠的影响。
材料和方法
40 只抑郁样模型大鼠通过强迫游泳试验（FST）、旷场试验（OFT）和蔗糖偏好试验（SPT）进行评估。模型大鼠分为五组：CUMS（n=8）、CAVOh+CUMS（n=8）、CAVOl+CUMS（n=8）、LVO+CUMS（n=8）和 OBI+CAVO+CUMS（n=8）。CUMS 诱导的大鼠接受为期 4 周的治疗。其他健康大鼠被视为对照（CTR，n=8）。采用酶联免疫吸附试验（ELISA）测定 CUMS 诱导大鼠脑组织匀浆中 5-羟色胺（5-HT）和多巴胺（DA）及其代谢物同型香草酸（HVA）和 5-羟吲哚乙酸（5-HIAA）的水平。
结果
CAVO 改善了抑郁样行为（p<0.05）。CUMS 组的 DA 水平低于 CTR 和 CAVOh 组（**p<0.01 和 *p<0.05）。CUMS 组的 HVA 水平低于 CTR、LVO、OBI+CAVOh 和 CAVOh 组（**p<0.01 和 *p<0.05），低于 OBI+CAVOh 组（**p<0.01）。CUMS 组的 5-HT 水平低于 CTR 和 CAVOh 组（**p<0.01）。CUMS 和 OBI+CAVOh 组的 5-HIAA 水平低于 CTR、LVO 和 CAVOh 组（**p<0.01）。
结论
CAVO 可改善 CUMS 诱导大鼠的抑郁样行为，并调节大脑中 DA 和 5-HT 的代谢。
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