Lucas P A, Dziewiatkowski D D
Department of Oral Biology, University of Michigan, Ann Arbor 48109-1078.
Connect Tissue Res. 1987;16(4):323-41. doi: 10.3109/03008208709005618.
The syntheses of proteoglycans and proteins by chondrocytes from the Swarm rat chondrosarcoma in primary cultures were modulated on the addition of matrical molecules. In the presence of hyaluronan, collagen or proteoglycan aggregates the synthesis of proteoglycans was depressed. The synthesis of collagen was also depressed in the presence of hyaluronan or collagen. In the presence of proteoglycan monomers, the incorporation of 35S-sulfate was enhanced in proportion to the concentration of the additive in the medium; the synthesis of protein was unaffected. The proteoglycan monomers synthesized in the presence of proteoglycan monomers were larger than those synthesized in their absence. In combinations, the exogenous macromolecules did not affect the selected biosynthetic activities to an extent greater than that which they exerted separately. The data suggest, however, that the proteoglycan monomers can counteract the inhibitory effects of the macromolecules which are inhibitory. The data, moreover, suggest that the chondrocytes of the Swarm rat chondrosarcoma have the potential to discriminate between proteoglycan monomers produced by self and those produced by chondrocytes of hyaline cartilages.
在原代培养中，添加基质分子可调节来自斯沃姆大鼠软骨肉瘤的软骨细胞对蛋白聚糖和蛋白质的合成。在透明质酸、胶原蛋白或蛋白聚糖聚集体存在的情况下，蛋白聚糖的合成受到抑制。在透明质酸或胶原蛋白存在的情况下，胶原蛋白的合成也受到抑制。在蛋白聚糖单体存在的情况下，35S-硫酸盐的掺入量与培养基中添加剂的浓度成正比增加；蛋白质的合成不受影响。在蛋白聚糖单体存在下合成的蛋白聚糖单体比不存在时合成的更大。组合使用时，外源性大分子对所选生物合成活性的影响程度并不大于它们单独作用时的影响。然而，数据表明蛋白聚糖单体可以抵消具有抑制作用的大分子的抑制作用。此外，数据表明斯沃姆大鼠软骨肉瘤的软骨细胞有潜力区分自身产生的蛋白聚糖单体和透明软骨软骨细胞产生的蛋白聚糖单体。
