在微团培养系统中评估转化生长因子-β3对人关节软骨细胞蛋白聚糖产生的影响。

Assessment of TGF-β3 on production of aggrecan by human articular chondrocytes in pellet culture system.

作者信息

Zamani Saeed, Hashemibeni Batool, Esfandiari Ebrahim, Kabiri Azadeh, Rabbani Hossein, Abutorabi Roshanak

机构信息

Department of Anatomical Sciences and Molecular Biology, Medical Faculty, Isfahan University of Medical Sciences, Iran.

Department of Biophysics, Medical Faculty, Isfahan University of Medical Sciences, Iran.

出版信息

Adv Biomed Res. 2014 Jan 27;3:54. doi: 10.4103/2277-9175.125799. eCollection 2014.

BACKGROUND

The Autologous Chondrocytes Transplantation (ACT) method is being studied for repair of cartilage diseases. As the chondrocytes dedifferentiated during monolayer culture, three-dimensional cultures are suggested to redifferentiate them. The aim of this study was investigation of the effect of TGF-β3 growth factor on chondrocytes in pellet culture system.

MATERIALS AND METHODS

The chondrocytes were isolated from three human articular cartilages by enzymatic digestion. The cells of the second passage were transferred to pellet culture system. We determined the chondrogenic medium with TGF-β3 as the experimental group and without it as the control group. After 2 weeks, the aggrecan production was investigated using histological and immunohistochemical (IHC) methods.

RESULTS

The presence of glycosaminoglycans was proved through Toluiden blue staining. Comparison of IHC results using MATLAB software showed that aggrecan in the experimental group was significantly higher than in the control group (P ≤ 0.05).