Unidad Monterrey, Centro de Investigación y de Estudios Avanzados, Vía del Conocimiento 201, PIIT, Autopista al Aeropuerto, Km. 9.5, Apodaca, Nuevo León, Mexico.
Unidad Monterrey, Centro de Investigación y de Estudios Avanzados, Vía del Conocimiento 201, PIIT, Autopista al Aeropuerto, Km. 9.5, Apodaca, Nuevo León, Mexico.
Sci Total Environ. 2019 Nov 15;691:278-283. doi: 10.1016/j.scitotenv.2019.06.538. Epub 2019 Jul 2.
Local anaesthetics are among the most used drugs in clinical practice, but once they are released to the environment, the effects on the aquatic fauna remain uncertain. This study evaluates, for the first time, the impact of tetracaine, lidocaine and bupivacaine on the survival rate and physiological effects of cladocera Daphnia magna. Video-tracking and image processing allowed us to obtain changes in behaviour parameters like swimming average velocity and mean square displacement. We found that tetracaine shows the most severe effect. A high-speed microscopy system was also used to determine the response of D. magna heart to these drugs. Our results show that tetracaine presents dose-dependent area reduction during all cardiac cycle, while bupivacaine and lidocaine did not present significative effects on heart size. The tested drugs, at environmental high concentrations, altered behaviour, heart function and survival of D. magna.
局部麻醉剂是临床实践中使用最广泛的药物之一，但一旦它们释放到环境中，对水生动物群的影响仍然不确定。本研究首次评估了丁卡因、利多卡因和布比卡因对枝角类动物大型溞存活率和生理效应的影响。视频跟踪和图像处理使我们能够获得游泳平均速度和均方根位移等行为参数的变化。我们发现，丁卡因的影响最为严重。我们还使用高速显微镜系统来确定大型溞心脏对这些药物的反应。我们的结果表明，丁卡因在整个心动周期中呈现出剂量依赖性的面积减小，而布比卡因和利多卡因对心脏大小没有显著影响。在环境高浓度下，测试药物改变了大型溞的行为、心脏功能和存活率。
