Rotllant Guiomar, Llonch Pol, García Del Arco José A, Chic Òscar, Flecknell Paul, Sneddon Lynne U
Institut de Ciències del Mar, Spanish National Research Council (CSIC), Passeig Marítim de la Barceloneta, 37, 08003 Barcelona, Spain.
Department of Animal and Food Science, Universitat Autònoma de Barcelona, Travessera dels Turons, s/n, Cerdanyola del Vallès, 08193 Barcelona, Spain.
Biology (Basel). 2023 Feb 28;12(3):387. doi: 10.3390/biology12030387.
Methods to induce analgesia and anesthesia for research purposes, handling, transport, or stunning have been used in 71 species of crustaceans. A non-systematic literature search was conducted on crustacean anesthetic methods. This review presents a comprehensive evaluation of drugs and non-chemical methods used to provide analgesia and anesthesia in many crustacean species rather than just decapod crustaceans. This information allows users to select an appropriate method or agent for their species of interest. We prepared an on-line tool based on datasette, a no-code open-source solution for simple web-based database frontends that allows exploration and downloading data by method, analgesic/anesthetic, species, life stage, or sex, as well as other data including environmental conditions (temperature, salinity, light), route of administration, dosage, and induction and recovery times. These values can be selected to filter the dataset and export it to CSV or JSON formats. Currently, several techniques and chemicals are, in our opinion, unsuitable for use as anesthetics in crustaceans, and the basis for these opinions are presented. Given the evidence of a pain-like experience in crustaceans, we propose that researchers should treat crustaceans humanely, applying the principles of good handling, care, and the management of stress and pain to safeguard their welfare.
用于研究目的、处理、运输或致昏的镇痛和麻醉方法已在71种甲壳类动物中使用。我们对甲壳类动物麻醉方法进行了非系统性文献检索。本综述全面评估了用于多种甲壳类动物（而非仅十足目甲壳类动物）提供镇痛和麻醉的药物及非化学方法。这些信息有助于使用者为其感兴趣的物种选择合适的方法或药剂。我们基于datasette编写了一个在线工具，datasette是一种无代码的开源解决方案，用于简单的基于网络的数据库前端，可按方法、镇痛/麻醉剂、物种、生命阶段或性别，以及包括环境条件（温度、盐度、光照）、给药途径、剂量、诱导和恢复时间等其他数据进行数据探索和下载。可以选择这些值来筛选数据集并将其导出为CSV或JSON格式。目前，我们认为有几种技术和化学物质不适用于甲壳类动物麻醉，并阐述了这些观点的依据。鉴于有证据表明甲壳类动物会有类似疼痛的体验，我们建议研究人员应人道地对待甲壳类动物，应用良好的处理、护理以及应激和疼痛管理原则来保障它们的福利。
