Integrating Biological Early Warning Systems with High-Resolution Online Chemical Monitoring in Wastewater Treatment Plants.

Detection of micropollutants (MPs) in wastewater effluents using traditional toxicity tests or chemical analysis with discrete samples is challenging due to concentration dynamics. This study evaluates a continuous monitoring approach for detecting MPs in wastewater effluents using a combination of biological early warning systems (BEWS). Three BEWS with , , and were operated in parallel in a full-scale municipal wastewater treatment plant. Concentrations of MPs were monitored by simultaneous online chemical analysis using high performance liquid chromatography-high resolution mass spectrometry (MS2Field). Over 5 weeks, behavioral changes observed in the BEWS occasionally exceeded acute toxicity thresholds, triggering alarms. These changes were related to MPs identified by the MS2Field, to abiotic factors, or to operational parameters of the BEWS. For one toxic event, behavioral responses were linked to a pesticide, not authorized in Switzerland, at concentrations close to literature EC values. Verification tests confirmed that the pesticide in the effluent was the most likely cause for the organism response. The study demonstrates the potential of BEWS as a stand-alone technique for detecting contamination peaks in wastewater, and identifies key limitations and critical factors that need to be addressed to optimize the use of BEWS in wastewater monitoring.