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固定化斜生栅藻的二氧化碳固定与铵摄取及其用于蛋白质生产的潜力。

Carbon dioxide fixation coupled with ammonium uptake by immobilized Scenedesmus obliquus and its potential for protein production.

机构信息

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, PR China.

State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, PR China.

出版信息

Bioresour Technol. 2019 Oct;289:121685. doi: 10.1016/j.biortech.2019.121685. Epub 2019 Jun 20.

Abstract

In this study, immobilized Scenedesmus obliquus (S. obliquus) was proposed to simultaneously alleviate the carbon dioxide (CO) and ammonium (NH-N). Two trophic modes of autotrophy and mixotrophy were conducted by batch experiments with a period of 5 days. The results shown that NH-N could be removed more efficiently if algal cells were immobilized, and the trophic mode change had no significant effect on immobilized S. obliquus to NH-N removal under 5% CO sparging. Specifically, immobilized S. obliquus could remove NH-N completely at initial concentrations of 30 and 50 mg/L and reached about 80% removal rate of NH-N at the concentration of 70 mg/L under both trophic modes. The protein synthesis was its main removal mechanism and the dominant amino acid components including glutamic acid (Glu), cystine (Cys), arginine (Arg), methionine (Met) and lysine (Lys) were sensitive to NH-N assimilation.

摘要

在这项研究中,拟通过批式实验(为期 5 天),利用固定化斜生栅藻(S. obliquus)同时缓解二氧化碳(CO)和氨氮(NH-N)。采用自养和混合营养两种营养方式。结果表明,如果藻类细胞被固定化,NH-N 的去除效率会更高,并且在 5% CO 鼓泡条件下,营养方式的变化对固定化斜生栅藻去除 NH-N 没有显著影响。具体而言,在两种营养方式下,固定化斜生栅藻均可在初始浓度为 30 和 50mg/L 时完全去除 NH-N,在 70mg/L 浓度下,NH-N 的去除率约为 80%。蛋白质合成是其主要的去除机制,而包括谷氨酸(Glu)、半胱氨酸(Cys)、精氨酸(Arg)、蛋氨酸(Met)和赖氨酸(Lys)在内的主要氨基酸组分对 NH-N 同化较为敏感。

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