Introduction of novel species will highlight technical feasibility of microalgae-based biofuels for commercial applications. This paper reports the effect of culture medium and light wavelength on biomass and fatty acid production of S. bacillaris which holds some advantages as short life cycle, easy cultivation, high lipid content, diversity of fatty acids and stability under harsh environmental conditions. The results displayed that, soil extract (SE) greatly enhance growth rate of cultures. Maximum biomass and lipid productivity were achieved in TAP medium as 81 mg/L·day, 19.44 mg/L·day; respectively. Light wavelength didn't significantly change growth kinetics but played a critical role on chlorophyll-a accumulation. C14:0, C16:0 and C18:0 fatty acids were abundant which are suitable for biodiesel conversion. Interestingly, blue and red light increased longer chain fatty acids content. These results indicated that; S. bacillaris holds potential for further development of biodiesel production and feasibility of algal biodiesel for fundamental and applied sciences.