温度及其与高光强组合对来自半干旱沙漠地区的单胞藻 Y2 产脂的影响。
Effects of temperature and its combination with high light intensity on lipid production of Monoraphidium dybowskii Y2 from semi-arid desert areas.
机构信息
Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Environmental Microbial Technology Center of Hubei Province, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan 430062, China.
Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
出版信息
Bioresour Technol. 2018 Oct;265:407-414. doi: 10.1016/j.biortech.2018.06.044. Epub 2018 Jun 15.
Temperature and light intensity are important environmental factors influencing microalgae for biodiesel production. The aim of present work was to study the effects of temperature (15 °C, 25 °C, and 35 °C) and its combination with high light intensity (HL, 400 μmol photon m s) on lipid production of Monoraphidium dybowskii Y2 which was isolated from desert. The results demonstrated that algal growth was only inhibited at 15 °C. Promoted lipid content and decreased Fv/Fm were observed in 15 °C and 35 °C. Cellular carbohydrate, protein conversion and membrane lipid (MGDG, DGDG and SQDG) remodeling contributes for lipid accumulation. Stress combined temperatures with HL are benefit for lipid production, especially desired neutral lipid productivity all exceed 40 mg L d. Fatty acids compositions of C16:0 and C18:1 were further promoted under 15 °C or 35 °C combined with HL. Thus, M. dybowskii Y2 will well-adapted to outdoors cultivation for biodiesel production.
温度和光照强度是影响微藻生物柴油生产的重要环境因素。本研究的目的是研究温度（15°C、25°C 和 35°C）及其与高光强（HL，400µmol 光子 m-2 s-1）组合对从沙漠中分离出的 Monoraphidium dybowskii Y2 产脂的影响。结果表明，只有在 15°C 时藻类生长才受到抑制。在 15°C 和 35°C 下观察到脂质含量增加和 Fv/Fm 降低。细胞碳水化合物、蛋白质转化和膜脂质（MGDG、DGDG 和 SQDG）重塑有助于脂质积累。胁迫将温度与 HL 结合有利于脂质生产，特别是所需中性脂质生产力均超过 40mgL-1d。在 15°C 或 35°C 与 HL 结合下，C16:0 和 C18:1 的脂肪酸组成进一步得到促进。因此，M. dybowskii Y2 将很好地适应户外生物柴油生产。
Zotero 插件