Papapanagiotou Georgia, Samara Christina, Psachoulia Paraskevi, Chatzidoukas Christos
Laboratory of Biochemical and Biotechnological Processes (LB²P), Department of Chemical Engineering, Aristotle University of Thessaloniki (AUTH), Thessaloniki, 54124, Greece.
World J Microbiol Biotechnol. 2024 Dec 18;41(1):12. doi: 10.1007/s11274-024-04229-y.
In this study, four microalgal strains were evaluated for their biomass production capacity and macromolecule biosynthesis. These include three strains from the phylum Chlorophyta: Monoraphidium sp. LB2PC 0120, Stichococcus sp. LB2PC 0117, and Tetraselmis sp. LB2PC 0320, and one strain from the phylum Haptophyta: Isochrysis sp. LB2PC 0220. The experiments were conducted under typical laboratory-scale setups. Additionally, phylogenetic analysis based on the 18-28 S rRNA internal transcribed spacer (ITS) was performed to validate the taxonomic identity of the strains. Each strain was exposed to four different cultivation conditions based on two levels of illumination intensity [25-(LI) and 50-(HI) µmol m s] and nitrogen loading [100-(LΝ) and 300-(HΝ) mg NaNO L] in a full factorial design. All the microalgae achieved maximum biomass production under HI-HN conditions, which amounted to 1495, 919, 844, and 708 mg/L for Monoraphidium sp. LB2PC 0120, Stichococcus sp. LB2PC 0117, Tetraselmis sp. LB2PC 0320 and Isochrysis sp. LB2PC 0220, respectively, after 16 days of cultivation. Among them, Stichococcus sp. LB2PC 0117 had the highest protein content (49.9% wt.) under LI-HN conditions and Monoraphidium sp. LB2PC 0120 had the highest lipid content (44.3% wt.) under HI-LN conditions. Both Monoraphidium sp. LB2PC 0120 and Tetraselmis sp. LB2PC 0320 accumulated the highest carbohydrate content (~ 37% wt.) under LI-LN and HI-LN conditions, respectively. Based on biomass and macromolecule production, Monoraphidium sp. LB2PC 0120 was identified as the most promising candidate for upscaling studies, expecting its highly manipulatable compositional profile to support multiple applications in the food industry, rendering this microalga a valuable resource.
在本研究中，评估了四种微藻菌株的生物质生产能力和大分子生物合成。其中包括绿藻门的三种菌株：单针藻属LB2PC 0120、针形藻属LB2PC 0117和四爿藻属LB2PC 0320，以及定鞭藻门的一种菌株：等鞭金藻属LB2PC 0220。实验在典型的实验室规模设置下进行。此外，基于18 - 28 S rRNA内部转录间隔区（ITS）进行了系统发育分析，以验证菌株的分类身份。在全因子设计中，根据光照强度[25 -（低光照，LI）和50 -（高光照，HI）μmol m⁻² s⁻¹]和氮负荷[100 -（低氮，LN）和300 -（高氮，HN）mg NaNO₃ L⁻¹]的两个水平，将每种菌株暴露于四种不同的培养条件下。所有微藻在高光照 - 高氮条件下均实现了最大生物质产量，培养16天后，单针藻属LB2PC 0120、针形藻属LB2PC 0117、四爿藻属LB2PC 0320和等鞭金藻属LB2PC 0220的产量分别达到1495、919、844和708 mg/L。其中，针形藻属LB2PC 0117在低光照 - 高氮条件下蛋白质含量最高（49.9%重量），单针藻属LB2PC 0120在高光照 - 低氮条件下脂质含量最高（44.3%重量）。单针藻属LB2PC 0120和四爿藻属LB2PC 0320分别在低光照 - 低氮和高光照 - 低氮条件下积累了最高的碳水化合物含量（约37%重量）。基于生物质和大分子生产，单针藻属LB2PC 0120被确定为扩大规模研究中最有前景的候选菌株，预计其高度可调控的成分特征将支持在食品工业中的多种应用，使这种微藻成为一种有价值的资源。
