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利用特定细菌和酵母控制鲜活饵料中的……

The Use of Selected Bacteria and Yeasts to Control . in Live Food.

作者信息

Sahandi Javad, Sorgeloos Partick, Xiao Hui, Wang Xianghong, Qi Zizhong, Zheng Yanfen, Tang Xuexi

机构信息

College of Marine Life Science, Ocean University of China, Qingdao 266100, China.

Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory of Marine Science and Technology, Qingdao 266071, China.

出版信息

Antibiotics (Basel). 2019 Jul 18;8(3):95. doi: 10.3390/antibiotics8030095.

Abstract

species are a significant causative of mass mortality in mariculture worldwide, which can quickly accumulate in live food and transmit into the larval gut. With restrictions on the use of antibiotics in aquaculture, finding a proper solution to reduce the risk of Vibriosis is vital. This study aimed to evaluate the susceptibility of Vibrio harveyi, V. campbellii, V. anguillarum, and V. parahaemolyticus to twenty-six bacterial and yeast strains and use the beneficial ones to enrich live food (Branchiopod, , rotifer, and copepod, ). Thus, a modified disk diffusion method was applied. After a susceptibility assay, the bacteria and yeast beneficial in suppressing the species were labeled by fluorescent stain and used to measure the accumulation potential in different live foods. Also, the beneficial bacteria and yeast were used to enrich live foods, and then the count of loaded was estimated after 5, 10, 15, and 20 hours by the serial dilution method. From the total bacteria and yeast strains that were used, Candida parapsilosis, Pseudoalteromonas and inhibited all four species. The results of microbial labeling showed that in , in rotifers, and in copepods had the highest accumulation rate. The results of the estimation of loaded in different live foods also showed that the use of beneficial bacteria and yeast each significantly reduced the count of . Application of bacteria and yeast to suppress pathogenic maybe a sustainable method for preventing this pathogen from harmfully invading aquaculture and may also aid in reducing the chances of antibiotic resistance in pathogenic .

摘要

弧菌是全球海水养殖中大规模死亡的重要致病菌,可在活饵中迅速积累并传播至幼体肠道。随着水产养殖中抗生素使用的限制,找到适当的解决方案以降低弧菌病风险至关重要。本研究旨在评估哈维氏弧菌、坎氏弧菌、鳗弧菌和副溶血性弧菌对26种细菌和酵母菌株的敏感性,并利用有益菌株来强化活饵(鳃足类、轮虫和桡足类)。因此,采用了改良的纸片扩散法。在敏感性测定后,对抑制弧菌的有益细菌和酵母用荧光染料标记,并用于测定在不同活饵中的积累潜力。此外,用有益细菌和酵母强化活饵,然后通过系列稀释法在5、10、15和20小时后估计弧菌的负载量。在所使用的全部细菌和酵母菌株中,近平滑念珠菌、假交替单胞菌和抑制了所有四种弧菌。微生物标记结果表明,在鳃足类中、在轮虫中和在桡足类中的积累率最高。不同活饵中弧菌负载量的估计结果还表明,使用有益细菌和酵母均显著降低了弧菌数量。应用细菌和酵母抑制致病性弧菌可能是一种可持续的方法,可防止该病原体对水产养殖造成有害侵袭,也有助于减少致病性弧菌产生抗生素耐药性的机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d0d/6784084/0de481f8b62a/antibiotics-08-00095-g001.jpg

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