Key Laboratory of Maricultural Organism Disease Control, Yellow Sea Fisheries Research Institute, Chinese Academic of Fishery Sciences, Qingdao 266071, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Mar Drugs. 2023 Mar 31;21(4):228. doi: 10.3390/md21040228.
spp. could be one of the most suitable substitutes for the control and prevention of aquatic diseases. The occurrence of species population, antimicrobial character, and virulence diversity in spp. recovered from the mariculture system in China between 2009 and 2021 were investigated, screening for probiotic strains with good biological safety that can inhibit , , , , . The results showed that 116 isolates were divided into 24 species, and the top three species were (37/116), (28/116), and (10/116). Among the 116 isolates, 32.8% were effective against , 30.1% for , 60.3% for , 69.8% for and 74.1% for . More than 62% of isolates were susceptible to florfenicol, doxycycline and tetracycline, etc., and 26/116 isolates were found to be multiple-antibiotic-resistant (MAR), with MARI values ranging from 0 to 0.06. Eighteen kinds of antibiotic resistance genes were tested; only , , and were detected. And 9 isolates in 2 species were excluded by 6/10 kinds of -related toxin gene (, , , , , ). Bio-safety testing indicated that three kinds of probiotics were good probiotic candidates to prevent Vibriosis. These results provide comprehensive genetic diversity, potential risks, and probiotic characteristics of in the mariculture system in China, and provide basic support for green and healthy development of aquatic industry.
种可能是控制和预防水产疾病的最适宜替代品之一。本研究于 2009 年至 2021 年期间调查了中国水产养殖系统中回收的种的物种种群、抗菌特性和毒力多样性，筛选出具有良好生物安全性、能抑制、、、、的益生菌株。结果表明，从分离出 116 株，分为 24 个种，前三种分别为（37/116）、（28/116）和（10/116）。在 116 株中，32.8%对有效，30.1%对有效，60.3%对有效，69.8%对有效，74.1%对有效。超过 62%的分离株对氟苯尼考、多西环素和四环素等敏感，发现 26/116 株为多重耐药（MAR），MARI 值在 0 到 0.06 之间。测试了 18 种抗生素耐药基因，仅检测到、和。在 2 个种的 9 株中，通过 6/10 种 -相关毒素基因（、、、、、）被排除在外。生物安全性测试表明，有 3 种益生菌是预防弧菌病的良好益生菌候选株。这些结果为中国水产养殖系统中的种提供了全面的遗传多样性、潜在风险和益生菌特性，为水产养殖业的绿色健康发展提供了基础支持。
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