一种已建立的 HPLC-MS/MS 方法，用于评估盐炙对菟丝子中六种成分药代动力学的影响。
An Established HPLC-MS/MS Method for Evaluation of the Influence of Salt Processing on Pharmacokinetics of Six Compounds in Cuscutae Semen.
机构信息
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
出版信息
Molecules. 2019 Jul 9;24(13):2502. doi: 10.3390/molecules24132502.
A sensitive and effective method was developed for clarifying the pharmacokinetic properties of six compounds (including hyperin, chlorogenic acid, neochlorogenic acid, p-coumaric acid, astragalin, and isoquercitrin) in two processed Cuscutae Semen samples by high performance liquid chromatography mass spectrometry (HPLC-MS/MS). The six compounds were separated by acetonitrile and 0.1% formic acid-water on an Agilent Eclipse plus C18 column (4.6 mm × 100 mm, 1.8 μm). All compounds were analyzed with negative ion mode in multiple reaction monitoring (MRM). The lower limits of quantification (LLOQ) of hyperin, astragalin, neochlorogenic acid, chlorogenic acid, isoquercitrin, and p-coumaric acid were 1, 0.1, 4, 0.1, 2, and 4 ng·mL, respectively. The validated approach was effectively used for the pharmacokinetics of six compounds of two processed Cuscutae Semen samples after oral administration to rat. The results indicated that salt processing could improve the adsorption and bioavailability of astragalin in Cuscutae Semen.
建立了一种灵敏、有效的高效液相色谱-串联质谱（HPLC-MS/MS）法，用于澄清两种炮制菟丝子样品中 6 种化合物（包括金丝桃苷、绿原酸、新绿原酸、对香豆酸、牡荆素和异槲皮苷）的药代动力学特性。这 6 种化合物在 Agilent Eclipse plus C18 柱（4.6mm×100mm，1.8μm）上，用乙腈和 0.1%甲酸-水分离。所有化合物均采用负离子模式多反应监测（MRM）进行分析。金丝桃苷、牡荆素、新绿原酸、绿原酸、异槲皮苷和对香豆酸的定量下限（LLOQ）分别为 1、0.1、4、0.1、2 和 4ng·mL。该验证方法有效地用于口服两种炮制菟丝子样品后 6 种化合物的药代动力学研究。结果表明，盐炙可提高菟丝子中牡荆素的吸收和生物利用度。
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