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虹彩病毒感染虹鳟红细胞的转录组和蛋白质组综合分析指向新型免疫调节靶点。

Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets.

作者信息

Nombela Ivan, Lopez-Lorigados Marina, Salvador-Mira Maria Elizabeth, Puente-Marin Sara, Chico Veronica, Ciordia Sergio, Mena Maria Carmen, Mercado Luis, Coll Julio, Perez Luis, Ortega-Villaizan Maria Del Mar

机构信息

Departamento de Bioquímica y Biología Molecular, Instituto de Biología Molecular y Celular (IBMC) and Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández (UMH), 03202 Elche, Spain.

Unidad de Proteómica, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain.

出版信息

Vaccines (Basel). 2019 Jul 9;7(3):63. doi: 10.3390/vaccines7030063.

DOI:10.3390/vaccines7030063
PMID:31324030
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6789484/
Abstract

Teleost red blood cells (RBCs) are nucleated and therefore can propagate cellular responses to exogenous stimuli. RBCs can mount an immune response against a variety of fish viruses, including the viral septicemia hemorrhagic virus (VHSV), which is one of the most prevalent fish viruses resulting in aquaculture losses. In this work, RBCs from blood and head kidney samples of rainbow trout challenged with VHSV were analyzed via transcriptomic and proteomic analyses. We detected an overrepresentation of differentially expressed genes (DEGs) related to the type I interferon response and signaling in RBCs from the head kidney and related to complement activation in RBCs from blood. Antigen processing and presentation of peptide antigen was overrepresented in RBCs from both tissues. DEGs shared by both tissues showed an opposite expression profile. In summary, this work has demonstrated that teleost RBCs can modulate the immune response during an in vivo viral infection, thus implicating RBCs as cell targets for the development of novel immunomodulants.

摘要

硬骨鱼的红细胞有细胞核，因此能够对外源刺激产生细胞反应。红细胞可以对多种鱼类病毒产生免疫反应，包括病毒性败血症出血病毒（VHSV），它是导致水产养殖损失的最常见鱼类病毒之一。在这项研究中，通过转录组学和蛋白质组学分析，对感染VHSV的虹鳟鱼血液和头肾样本中的红细胞进行了分析。我们检测到，头肾红细胞中与I型干扰素反应和信号传导相关的差异表达基因（DEG）显著富集，血液红细胞中与补体激活相关的DEG显著富集。两个组织的红细胞中，肽抗原的抗原加工和呈递均显著富集。两个组织共有的DEG表现出相反的表达谱。总之，这项研究表明，硬骨鱼红细胞可以在体内病毒感染期间调节免疫反应，因此表明红细胞是新型免疫调节剂开发的细胞靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/044c397beec5/vaccines-07-00063-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/a4c9e353b7dc/vaccines-07-00063-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/3a093b0b28f2/vaccines-07-00063-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/044c397beec5/vaccines-07-00063-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/12828ad86eef/vaccines-07-00063-g001a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/cc2a07dd3ca6/vaccines-07-00063-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/b1d9601b4236/vaccines-07-00063-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/5f8dbecbd74e/vaccines-07-00063-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/a4c9e353b7dc/vaccines-07-00063-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/3a093b0b28f2/vaccines-07-00063-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c4b1/6789484/044c397beec5/vaccines-07-00063-g007.jpg

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