College of Life Science, Liaoning Normal University, Dalian, 116081, China.
Lamprey Research Center, Liaoning Normal University, Dalian, 116081, China.
Fish Physiol Biochem. 2019 Dec;45(6):1829-1843. doi: 10.1007/s10695-019-00683-w. Epub 2019 Jul 19.
CDK-2, a member of the cyclin-dependent kinase family, plays an important role in many cell processes, such as cell cycle regulation, cell growth and differentiation, and cell apoptosis. Lampreys belong to the most primitive vertebrates, and there is no report about the CDK-2 gene in lampreys at present. In this study, a CDK-2-like gene sequence and deduced amino acid sequence were identified in Japanese lamprey (Lampetra japonica, L. japonica). The CDK-2-like gene has about 80% similarity with its homologs in jaw vertebrates. The polyclonal antibody against CDK-2-like was well prepared, and the results showed that CDK-2-like was highly expressed in the gonad tissue of lampreys. Apoptosis could reduce the expression of CDK-2-like in lymphocytes of lamprey, while overexpression of CDK-2-like could inhibit apoptosis. In addition, inhibition of CDK-2-like activity was able to trigger out apoptosis and also helped apoptotic inducer actinomycin D (Act-D) to induce apoptosis. These results suggest that CDK-2-like identified from lamprey may play a crucial role in apoptosis of jawless vertebrates.
CDK-2 是细胞周期蛋白依赖性激酶家族的成员，在许多细胞过程中发挥重要作用，如细胞周期调控、细胞生长和分化以及细胞凋亡。七鳃鳗属于最原始的脊椎动物，目前尚无关于七鳃鳗 CDK-2 基因的报道。本研究在日本七鳃鳗（Lampetra japonica，L. japonica）中鉴定出了一个 CDK-2 样基因序列和推导的氨基酸序列。CDK-2 样基因与颌脊椎动物的同源物具有约 80%的相似性。针对 CDK-2 样的多克隆抗体被很好地制备出来，结果表明 CDK-2 样在七鳃鳗的性腺组织中高度表达。凋亡可以降低七鳃鳗淋巴细胞中 CDK-2 样的表达，而 CDK-2 样的过表达可以抑制凋亡。此外，抑制 CDK-2 样的活性可以引发凋亡，并且有助于凋亡诱导剂放线菌素 D（Act-D）诱导凋亡。这些结果表明，从七鳃鳗中鉴定出的 CDK-2 样可能在无颌脊椎动物的凋亡中发挥关键作用。
