Molina H A, Kierszenbaum F
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
J Parasitol. 1988 Jun;74(3):370-4.
We studied the kinetics of development of inflammation in the myocardium and skeletal muscles of mice infected with Trypanosoma cruzi by determining the numbers of mononuclear cells (MNC), neutrophils, and eosinophils at tissue sites with varying degrees of damage. In the myocardium, areas with incipient inflammation and preserved tissue had the smallest numbers of inflammatory cells, 96-100% of which were MNC. In lesions where inflammatory cells accumulated in interstitial spaces displacing myofibers, MNC were also predominant (greater than 98%) but were present in larger numbers than in areas with preserved tissue. The number of MNC was even larger in necrotic areas where there was also marked neutrophil infiltration at the time when amastigote nests were frequently present. In skeletal muscle, MNC were also the first cells to infiltrate lesion sites; their numbers increased with the degree of severity of the lesion. Neutrophil accumulation also accompanied skeletal muscle necrosis. A salient difference was eosinophil accumulation in the necrotic lesions of skeletal muscle but not in the myocardium. The results identify MNC as the cell that initiates the inflammatory process in the heart and skeletal muscles of T. cruzi-infected mice. In these tissues the number of MNC appeared to be a good correlate of lesion severity.
我们通过测定不同损伤程度组织部位的单核细胞（MNC）、中性粒细胞和嗜酸性粒细胞数量，研究了感染克氏锥虫的小鼠心肌和骨骼肌炎症发展的动力学。在心肌中，初期炎症且组织保存完好的区域炎症细胞数量最少，其中96 - 100%为MNC。在炎症细胞积聚于间质空间并取代肌纤维的病变区域，MNC也占主导（超过98%），但其数量比组织保存完好的区域更多。在坏死区域，当经常出现无鞭毛体巢时，MNC数量甚至更多，同时还伴有明显的中性粒细胞浸润。在骨骼肌中，MNC也是最早浸润病变部位的细胞；其数量随病变严重程度增加。中性粒细胞积聚也伴随骨骼肌坏死。一个显著差异是嗜酸性粒细胞在骨骼肌坏死病变中积聚，而在心肌中不积聚。结果表明MNC是引发克氏锥虫感染小鼠心脏和骨骼肌炎症过程的细胞。在这些组织中，MNC数量似乎与病变严重程度密切相关。
