Qingdao Engineering Research Center for Rural Environment, Qingdao Agricultural University, Qingdao 266109, China.
Qingdao Engineering Research Center for Rural Environment, Qingdao Agricultural University, Qingdao 266109, China.
Int J Biol Macromol. 2019 Oct 1;138:433-440. doi: 10.1016/j.ijbiomac.2019.07.105. Epub 2019 Jul 17.
Lignin peroxidase (LiP) was obtained from Pichia methanolica through heterologous expression. LiP was extracted, purified, and immobilized on FeO@SiO@polydopamine (PDA) nanoparticles to acquire immobilized LiP. The optimal preparation conditions for immobilized LiP were investigated. Results showed that the immobilization efficiency of immobilized LiP reached 56.37% when the enzyme amount, PDA concentration, and immobilization time were 12 mg, 1.6 mg/mL, and 12 h, respectively. Compared with free LiP, the immobilized LiP showed good thermal stability and storage stability and improved pH tolerance. It also retained more than 30% of its initial activity after 8 cycles, demonstrating its improved reusability. The immobilized LiP demonstrated efficacy of reaction of 100%, 100%, 100%, 100%, 79%, 73%, and 65% for tetracycline, dibutyl phthalate, 5-chlorophenol, phenol, phenanthrene, fluoranthene, and benzo(a)pyrene, respectively, while the inactivated immobilized LiP only adsorbed <25% of phenanthrene and fluoranthene. The dissipation of organic pollutants was a combination of degradation and adsorption, with the former playing a more important role.
木质素过氧化物酶（LiP）通过异源表达从甲醇毕赤酵母中获得。LiP 被提取、纯化并固定在 FeO@SiO@聚多巴胺（PDA）纳米粒子上，以获得固定化 LiP。研究了固定化 LiP 的最佳制备条件。结果表明，当酶量、PDA 浓度和固定化时间分别为 12mg、1.6mg/mL 和 12h 时，固定化 LiP 的固定化效率达到 56.37%。与游离 LiP 相比，固定化 LiP 具有良好的热稳定性和储存稳定性，提高了 pH 耐受性。在 8 次循环后，它仍保留了初始活性的 30%以上，表明其可重复使用性得到了提高。固定化 LiP 对土霉素、邻苯二甲酸二丁酯、5-氯苯酚、苯酚、菲、荧蒽和苯并(a)芘的反应效率分别为 100%、100%、100%、100%、79%、73%和 65%，而失活的固定化 LiP 对菲和荧蒽的吸附率均低于 25%。有机污染物的降解是降解和吸附的结合，前者起更重要的作用。
