Zhu Shiyu, Li Yuheng, Chen Xu, Zhu Zhenzhou, Li Shuyi, Song Jingxin, Zheng Zhiqiang, Cong Xin, Cheng Shuiyuan
School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University, 36 Huanhu Middle Road, Wuhan 430048, China.
Systems Engineering Institute, Beijing 100010, China.
Foods. 2024 Jun 3;13(11):1753. doi: 10.3390/foods13111753.
Enzymatically derived selenium-enriched peptides from (CV) can serve as valuable selenium supplements. However, the industrial application of free enzyme is impeded by its limited stability and reusability. Herein, this study explores the application of co-immobilized enzymes (Alcalase and Dispase) on amino resin for hydrolyzing CV proteins to produce selenium-enriched peptides. The successful enzyme immobilization was confirmed through scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and Fourier-transform infrared spectroscopy (FTIR). Co-immobilized enzyme at a mass ratio of 5:1 (Alcalase/Dispase) exhibited the smallest pore size (7.065 nm) and highest activity (41 U/mg), resulting in a high degree of hydrolysis of CV protein (27.2%), which was obviously higher than the case of using free enzymes (20.7%) or immobilized Alcalase (25.8%). In addition, after a month of storage, the co-immobilized enzyme still retained a viability level of 41.93%, showing fairly good stability. Encouragingly, the selenium-enriched peptides from co-immobilized enzyme hydrolysis exhibited uniform distribution of selenium forms, complete amino acid fractions and homogeneous distribution of molecular weight, confirming the practicality of using co-immobilized enzymes for CV protein hydrolysis.
从（CV）中酶法制备的富硒肽可作为有价值的硒补充剂。然而，游离酶的工业应用受到其有限的稳定性和可重复使用性的阻碍。在此，本研究探索了共固定化酶（碱性蛋白酶和中性蛋白酶）在氨基树脂上用于水解CV蛋白以生产富硒肽的应用。通过扫描电子显微镜（SEM）、能量色散X射线（EDX）和傅里叶变换红外光谱（FTIR）证实了酶的成功固定化。质量比为5:1（碱性蛋白酶/中性蛋白酶）的共固定化酶表现出最小的孔径（7.065 nm）和最高的活性（41 U/mg），导致CV蛋白的水解程度很高（27.2%），明显高于使用游离酶（20.7%）或固定化碱性蛋白酶（25.8%）的情况。此外，储存一个月后，共固定化酶仍保持41.93%的活力水平，显示出相当好的稳定性。令人鼓舞的是，共固定化酶水解得到的富硒肽表现出硒形态的均匀分布、完整的氨基酸组成和分子量的均匀分布，证实了使用共固定化酶水解CV蛋白的实用性。
